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Autophagy Protects the Proximal Tubule from Degeneration and Acute Ischemic Injury

2011; American Society of Nephrology; Volume: 22; Issue: 5 Linguagem: Inglês

10.1681/asn.2010070705

1533-3450

Tomonori Kimura, Yoshitsugu Takabatake, Atsushi Takahashi, Jun-ya Kaimori, Isao Matsui, Tomoko Namba‐Hamano, Harumi Kitamura, Fumio Niimura, Taiji Matsusaka, Tomoyoshi Soga, Hiromi Rakugi, Yoshitaka Isaka,

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Autophagy is a bulk protein degradation system that likely plays an important role in normal proximal tubule function and recovery from acute ischemic kidney injury. Using conditional Atg5 gene deletion to eliminate autophagy in the proximal tubule, we determined whether autophagy prevents accumulation of damaged proteins and organelles with aging and ischemic renal injury. Autophagy-deficient cells accumulated deformed mitochondria and cytoplasmic inclusions, leading to cellular hypertrophy and eventual degeneration not observed in wildtype controls. In autophagy-deficient mice, I/R injury increased proximal tubule cell apoptosis with accumulation of p62 and ubiquitin positive cytoplasmic inclusions. Compared with control animals, autophagy-deficient mice exhibited significantly greater elevations in serum urea nitrogen and creatinine. These data suggest that autophagy maintains proximal tubule cell homeostasis and protects against ischemic injury. Enhancing autophagy may provide a novel therapeutic approach to minimize acute kidney injury and slow CKD progression.