An EB1-Binding Motif Acts as a Microtubule Tip Localization Signal
2009; Cell Press; Volume: 138; Issue: 2 Linguagem: Inglês
10.1016/j.cell.2009.04.065
ISSN1097-4172
AutoresSrinivas Honnappa, Susana Montenegro Gouveia, Anke Weisbrich, Fred F. Damberger, Neel Sarovar Bhavesh, Hatim Jawhari, Ilya Grigoriev, Frederik J.A. van Rijssel, Rubén M. Buey, Aleksandra Lawera, Ilian Jelesarov, Fritz K. Winkler, Kurt Wüthrich, Anna Akhmanova, Michel O. Steinmetz,
Tópico(s)Cellular transport and secretion
ResumoMicrotubules are filamentous polymers essential for cell viability. Microtubule plus-end tracking proteins (+TIPs) associate with growing microtubule plus ends and control microtubule dynamics and interactions with different cellular structures during cell division, migration, and morphogenesis. EB1 and its homologs are highly conserved proteins that play an important role in the targeting of +TIPs to microtubule ends, but the underlying molecular mechanism remains elusive. By using live cell experiments and in vitro reconstitution assays, we demonstrate that a short polypeptide motif, Ser-x-Ile-Pro (SxIP), is used by numerous +TIPs, including the tumor suppressor APC, the transmembrane protein STIM1, and the kinesin MCAK, for localization to microtubule tips in an EB1-dependent manner. Structural and biochemical data reveal the molecular basis of the EB1-SxIP interaction and explain its negative regulation by phosphorylation. Our findings establish a general “microtubule tip localization signal” (MtLS) and delineate a unifying mechanism for this subcellular protein targeting process.
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