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Electron staining of synaptic vesicles using the Champy-Maillet technique

1970; Wiley; Volume: 91; Issue: 2 Linguagem: Inglês

10.1111/j.1365-2818.1970.tb02211.x

1365-2818

Amanda Pellegrino de Iraldi, Ángela M. Suburo,

Mitochondrial Function and Pathology

SUMMARY Synaptic vesicles from rat pineal nerves can be stained by Champy‐Maillet mixtures containing osmium tetroxide and a soluble iodide. Two components of the vesicle (the matrix and the dense core) are revealed in a different way depending on at least two factors: the iodide employed and the final pH of the mixture. We have tried the following mixtures: zinc iodide at pH 5·5 (ZIO), cadmium iodide at pH 6·0 (CIO) and potassium iodide at pH 7·2 (KIO‐7) or pH 5·5 (KIO‐5). The matrix is more reactive with ZIO, while the dense core is more reactive with CIO and especially with KIO‐7. With the only difference of a lower pH, KIO‐5 stains the whole matrix of some vesicles and also reveals some tubular structures which are more frequently found in preterminal axons.