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Effect of cis -9, trans -11-conjugated linoleic acid on cell cycle of gastric adenocarcinoma cell line (SGC-7901)

2002; Baishideng Publishing Group; Volume: 8; Issue: 2 Linguagem: Inglês

10.3748/wjg.v8.i2.224

2219-2840

Jia‐Ren Liu, LI Bai-xiang, Bingqing Chen, Xiao-Hui Han, Yingben Xue, Yanmei Yang, Zheng Yu-mei, Ruihai Liu,

Cancer, Lipids, and Metabolism

AIM:To determine the effect of cis-9,trans-11conjugated linoleic acid (c9,t11-CLA) on the cell cycle of gastric cancer cells (SGC-7901) and its possible mechanism in inhibition cancer growth. METHODS:Using cell culture and immunocytochemical techniques, we examined the cell growth, DNA synthesis, expression of PCNA, cyclin A,B 1 ,D 1 ,P16 ink4a and p21 cip/waf1 of SGC-7901 cells which were treated with various c9,t11-CLA concentrations (25,50,100 and 200µ µ µ µ µmol•L -1 )of c9,t11-CLA for 24 and 48h, with a negative control (0.1% ethane). RESULTS:The cell growth and DNA synthesis of SGC-7901 cells were inhibited by c9,t11-CLA.SGC-7901 cells.Eight day after treatment with various concentrations of c9, t11-CLA mentioned above, the inhibition rates were 5. 92%,20.15%,75.61% and 82.44%,respectively and inhibitory effect of c9,t11-CLA on DNA synthesis (except for 25µ µ µ µ µmol/L,24h) showed significantly less 3 H-TdR incorporation than that in the negative controls (P<0.05 and P<0.01).Immunocytochemical staining demonstrated that SGC-7901 cells preincubated in media supplemented with different c9,t11-CLA concentrations at various times significantly decreased the expressions of PCNA (the expression rates were 7.2-3.0%,24hand 9.1-0.9% at 48h, respectively), Cyclin A (11.0-2.3%, 24h and 8.5-0.5%,48h),B1 (4.8-1.8% at 24h and 5.5-0.6% at 48h)and D 1 (3.6-1.4% at 24h and 3.7%-0 at 48h) as compared with those in the negative controls(the expressions of PCNA, Cyclin A, B1 and D1 were 6.5% at 24h and 9.0% at 48h, 4.2% at 24h and 5.1% at 48h, 9.5% at 24h and 6.0% at 48h,respectively)(P<0.01), whereas the expressions of p16 ink4a and p21 cip/waf1 , cyclindependent kinases inhibitors(CDKI), were increased. CONCLUSION:The cell growth and proliferation of SGC-7901 cell is inhibited by c9,t11-CLA via blocking the cell cycle, with reduced expressions of cyclin A,B 1 and D 1 and enhanced expressions of CDKI(p16 ink4a and p21 cip/waf1 ).