Identification of two new phylogenetically distant phytoplasmas from Senna surattensis plants exhibiting stem fasciation and shoot proliferation symptoms
2011; Wiley; Volume: 160; Issue: 1 Linguagem: Inglês
10.1111/j.1744-7348.2011.00517.x
ISSN1744-7348
AutoresW. Wu, Haiyuan Cai, Wei Wei, Robert E. Davis, I.‐M. Lee, H. Chen, Yan Zhao,
Tópico(s)Cocoa and Sweet Potato Agronomy
ResumoAbstract Sunshine trees ( S enna surattensis ) exhibiting unusual stem fasciation symptoms were observed in Y unnan, C hina. Morphological abnormalities of the affected plants included enlargement and flattening of stems and excessive proliferation of shoots. An electron microscopic investigation revealed presence of single membrane bound mycoplasma‐like bodies in sieve elements of symptomatic plants. With DNA templates extracted from diseased plants and phytoplasma universal primers P 1/ P 7 and P 1 A / R 16 S ‐ SR , nested polymerase chain reactions produced amplicons of 1.5 kb . Subsequent restriction fragment polymorphism and nucleotide sequence analyses of the amplicons indicated that the diseased plants were infected by distinct phytoplasmas affiliated with two phylogenetically distant taxa classified in two 16Sr groups (16 SrXII and 16 SrV ). This is the first report that sunshine tree is a natural host of two evolutionarily divergent phytoplasmas and the first report that a ‘ C andidatus Phytoplasma australiense’‐related strain is present in C hina. The findings signal a significant expansion of both geographical distribution and host range of 16 SrXII and 16 SrV phytoplasmas.
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