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BIBTEX RIS

Genetic diversity among native isolates of rhizobia from Phaseolus lunatus

2010; BioMed Central; Volume: 61; Issue: 3 Linguagem: Inglês

10.1007/s13213-010-0156-7

1869-2044

Jardel Oliveira Santos, Jadson Emanuel Lopes Antunes, Ademir Sérgio Ferreira de Araújo, Maria do Carmo Catanho Pereira de Lyra, Regina Lúcia Ferreira Gomes, Ângela Celis Almeida Lopes, Márcia do Vale Barreto Figueiredo,

Plant nutrient uptake and metabolism

The genetic diversity among native nodular rhizobia of Lima bean in soil from Piauí State, Brazil, was characterized and evaluated. The genotype UFPI-491 was used as a trap plant for rhizobia, with soil samples collected in Piaui State being used as the inoculum source. For isolation, nodules were collected at 45 days after seedling emergence—a period that presented the highest values for number and biomass of nodules evaluated in a previous experiment. In total, 50 isolates were obtained and placed into groups based on divergence from the morphological and physiological characterization. In general, the restriction patterns obtained with endonucleases MboI, HaeIII and NheI showed sufficient variability to discriminate between the isolates in this study. The main characteristics exhibited by isolates identified species from the genera Bradyrhizobium, Mesorhizobium and Rhizobium.