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Release of IL‐1 β via IL‐1 β ‐Converting Enzyme in a Skin Dendritic Cell Line Exposed to 2,4‐Dinitrofluorobenzene

2005; Hindawi Publishing Corporation; Volume: 2005; Issue: 3 Linguagem: Inglês

10.1155/mi.2005.131

1466-1861

Teresa J. Matos, Sara Jaleco, Margarida Gonçalo, Carlos B. Duarte, María Celeste Lopes,

Immune Response and Inflammation

We used a mouse fetal skin dendritic cell line (FSDC) to study the effect of the strong allergen 2,4‐dinitrofluorobenzene (DNFB) on interleukin (IL)‐1 β release and IL‐1 β receptor immunoreactivity. Stimulation with DNFB (30 minutes) increased IL‐1 β release without changing the mRNA levels of the protein. Furthermore, DNFB increased transiently the interleukin‐1 β ‐converting enzyme (ICE) activity, as measured with its fluorogenic substrate Z‐Tyr‐Val‐Ala‐Asp‐AFC. The ICE inhibitor Z‐YVAD‐FMK prevented the release of IL‐1 β evoked by DNFB. Incubation of the cells with DNFB (30 minutes) strongly increased IL‐1 β receptor immunoreactivity. The rapid effect of DNFB on the release of mature IL‐1 β , without inducing an increase of IL‐1 β mRNA in FSDC, suggests a posttranslational modification of pro‐IL‐1 β by ICE activity.