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In Vivo Transduction of an Stx-Encoding Phage in Ruminants

2006; American Society for Microbiology; Volume: 72; Issue: 7 Linguagem: Inglês

10.1128/aem.00157-06

1098-5336

Nancy A. Cornick, Amy F. Helgerson, Volker Mai, Jennifer M. Ritchie, David W. K. Acheson,

Bacterial Genetics and Biotechnology

ABSTRACT We assessed the ability of a kanamycin-marked Stx phage to move into a commensal, ovine Escherichia coli strain in the ruminant gastrointestinal tract. Transduction was detected in 19/24 sheep tested, resulting in the recovery of 47 transductants. Subtherapeutic doses of the quinolone antibiotic enrofloxacin did not increase the rate of transduction.