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Electrochemical Impedance Biosensor for Glucose Detection Utilizing a Periplasmic E. coli Receptor Protein

2005; Electrochemical Society; Volume: 8; Issue: 8 Linguagem: Inglês

10.1149/1.1943549

1944-8775

J. Wang, Kendra S. Carmon, L.A. Luck, Ian Ivar Suni,

Electrochemical sensors and biosensors

We report the development of a reagentless electrochemical impedance biosensor for glucose that employs the D-glucose∕galactose receptor from E. coli for direct glucose detection. The biological platform for this sensor is an Au surface to which the protein is immobilized through formation of an Au-S bond to a genetically engineered cysteine residue at the N-terminus. The impedance signal detects the extensive ligand-induced domain motion within the protein upon glucose binding. We show the applicability of impedance spectroscopy in conjunction with periplasmic binding proteins as a general method for detecting small molecules.