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Size of the Streptococcus mutans GS-5 chromosome as determined by pulsed-field gel electrophoresis

1990; American Society for Microbiology; Volume: 58; Issue: 3 Linguagem: Inglês

10.1128/iai.58.3.838-840.1990

1098-5522

John Tudor, Laura Marri, P. J. Piggot, L Daneo-Moore,

RNA and protein synthesis mechanisms

Rare cutting restriction endonucleases were used to cut the Streptococcus mutans chromosome into large fragments. Restriction enzymes utilizing recognition sites containing 6-, 7-, or 8-base-pair sequences with only G and C nucleotides produced few fragments, most of which were greater than 100 kilobase pairs in size. Addition of the fragments from digests of SmaI, NotI, ApaI, RsrII, and EagI yielded a molecular size for the S. mutans GS-5 genome of 2,819 +/- 60 kilobase pairs.