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BIBTEX RIS

Glutamate uptake is not a major target site for anaesthetic agents

1995; Elsevier BV; Volume: 75; Issue: 1 Linguagem: Inglês

10.1093/bja/75.1.61

ISSN

1471-6771

Autores

Beverley Nicol, David J. Rowbotham, David G. Lambert,

Tópico(s)

Anesthesia and Neurotoxicity Research

Resumo

We have examined the effects of thiopentone, propofol and ketamine 3-300 mumol litre-1, 3.6%, 2.4 rat MAC of isoflurane, 3.0%, 2.4 rat MAC of halothane and morphine 0.1-10 mumol litre-1 on uptake of [3H]glutamate into rat cerebrocortical and cerebellar synaptosomes. Corticol and cerebellar synaptosomes took up [3H]glutamate in a time-, concentration-, Na(+)-dependent and L-transpyrrolidine-2,4-dicarboxylate inhibitory manner. The Km and Vmax values for uptake were 8.6 mumol litre-1 and 1.7 nmol/min/mg protein and 2.2 mumol litre-1 and 0.7 nmol/min/mg protein in cortical and cerebellar preparations, respectively. At clinically relevant concentrations none of the agents tested influenced the uptake process. Our data suggest that the uptake of glutamate is not a major target site for anaesthetic or analgesic agents.

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