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Laser-induced choroidal neovascularization model to study age-related macular degeneration in mice

2013; Nature Portfolio; Volume: 8; Issue: 11 Linguagem: Inglês

10.1038/nprot.2013.135

1754-2189

Vincent Lambert, Julie Lecomte, Sylvain Hansen, Silvia Blacher, Maria-Luz Alvarez Gonzalez, Ingrid Struman, Nor Eddine Sounni, Eric Rozet, Pascal De Tullio, Jean Michel Foidart, Jean‐Marie Rakic, Agnès Noël,

Glaucoma and retinal disorders

The mouse model of laser-induced choroidal neovascularization (CNV) has been used extensively in studies of the exudative form of age-related macular degeneration (AMD). This experimental in vivo model relies on laser injury to perforate Bruch's membrane, resulting in subretinal blood vessel recruitment from the choroid. By recapitulating the main features of the exudative form of human AMD, this assay has served as the backbone for testing antiangiogenic therapies. This standardized protocol can be applied to transgenic mice and can include treatments with drugs, recombinant proteins, antibodies, adenoviruses and pre-microRNAs to aid in the search for new molecular regulators and the identification of novel targets for innovative treatments. This robust assay requires 7–14 d to complete, depending on the treatment applied and whether immunostaining is performed. This protocol includes details of how to induce CNV, including laser induction, lesion excision, processing and different approaches to quantify neoformed vasculature.