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Identification and characterization of a 30 kd major allergen from

1993; Elsevier BV; Volume: 92; Issue: 6 Linguagem: Inglês

10.1016/0091-6749(93)90061-j

1097-6825

R C Lin, Hong Shen, Seokmin Han,

Asthma and respiratory diseases

The allergenic components of the shrimp (Parapenaeus fissurus) were identified by immunoblotting, with sera from 10 allergic patients.Six components, ranging in molecular weight from about 86 to 39 kd, showed IgE-binding activity and were identified as allergens qf the shrimp.The component with a molecular weight of about 39 kd showed the highest frequency of IgE binding (70%) and was constdered to be one of its major allergens.Two monoclonal antibodies against this 39 kd component were generated, and their antigenic cross-reactivity with five different kimis of seafood, shrimp, crab, cuttlejish, oyster and pomfret was analyzed.Monoclonal antibody I-6-IOB reacted with the 39 kd component from shrimp only, but monoclonal antibody 2-7-IE also reacted with the 39 kd component from crab.By extraction with 0.5% sodium dodecykiulfate and ethanol precipitation, a highly purified shrimp 39 kd component was obtained.In two-dimensional gel electrophoresis six kofonns of this purified 39 kd component, with isoelectric point values from 5.1 to 5.6, were identified.No marked dijference was observed when the amino acid composition of this purified 39 kd allergen was compared with those of serum albumin from different animals.They all contain a high proportion of acidic amino acids.There was also a 62% to 83% sequence homology among three different pairs of peptide fragments of purified 39 kd components of shrimp and crab.In conclusion, a 39 kd major allergen from the shrimp has been identified and characterized in the present study.According to the suggestions of the International Union of Immunological