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Role of caveolin‐1 and cytoskeletal proteins, actin and vimentin, in adipogenesis of bovine intramuscular preadipocyte cells

2004; Wiley; Volume: 28; Issue: 8-9 Linguagem: Inglês

10.1016/j.cellbi.2004.05.003

1095-8355

Takato Takenouchi, Norikazu Miyashita, Kyohei Ozutsumi, Michael T. Rose, Hisashi Aso,

Metabolism, Diabetes, and Cancer

Abstract We investigated the involvement of caveolin‐1 and the cytoskeletal proteins, actin and vimentin, in the adipogenesis of bovine intramuscular preadipocyte (BIP) cells. Immunoblot analysis demonstrated that levels of caveolin‐1 and actin gradually increased during adipose conversion in BIP cells, whereas a slight decrease was observed for vimentin. We found that part of the vimentin was clearly distributed to caveolin‐1‐enriched membrane fractions in BIP cells, but actin was not. During adipogenesis of BIP cells, treatment with the tubulin depolymerizer, nocodazole, significantly increased intracellular triglyceride accumulation compared to non‐treated cells. Immunocytochemical analysis showed that actin microfilaments were significantly disrupted in nocodazole‐treated cells. Also, a decrease in the localization of vimentin in caveolin‐1‐enriched fractions and a failure of vimentin to co‐immunoisolate with caveolin‐1 were observed in nocodazole‐treated cells. These results suggest that a rearrangement of cytoskeletal proteins has a role in the intracellular accumulation of lipid droplets during adipogenesis of BIP cells.