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Involvement of aquaporin‐7 in the cutaneous primary immune response through modulation of antigen uptake and migration in dendritic cells

2011; Wiley; Volume: 26; Issue: 1 Linguagem: Inglês

10.1096/fj.11-186627

1530-6860

Mariko Hara‐Chikuma, Yoshinori Sugiyama, Kenji Kabashima, Eisei Sohara, Shinichi Uchida, Sei Sasaki, Shintaro Inoue, Yoshiki Miyachi,

Circadian rhythm and melatonin

The FASEB JournalVolume 26, Issue 1 p. 211-218 Research CommunicationFree to Read Involvement of aquaporin-7 in the cutaneous primary immune response through modulation of antigen uptake and migration in dendritic cells Mariko Hara-Chikuma, Corresponding Author Mariko Hara-Chikuma [email protected] Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, Japan Innovative Beauty Science Laboratory, Kanebo Cosmetics Inc., Odawara, JapanCorrespondence: Center for Innovation in Immunoregulative Technology and Therapeutics, Graduate School of Medicine, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan. E-mail: [email protected]Search for more papers by this authorYoshinori Sugiyama, Yoshinori Sugiyama Innovative Beauty Science Laboratory, Kanebo Cosmetics Inc., Odawara, JapanSearch for more papers by this authorKenji Kabashima, Kenji Kabashima Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, JapanSearch for more papers by this authorEisei Sohara, Eisei Sohara Department of Nephrology, Tokyo Medical and Dental University, Tokyo, JapanSearch for more papers by this authorShinichi Uchida, Shinichi Uchida Department of Nephrology, Tokyo Medical and Dental University, Tokyo, JapanSearch for more papers by this authorSei Sasaki, Sei Sasaki Department of Nephrology, Tokyo Medical and Dental University, Tokyo, JapanSearch for more papers by this authorShintaro Inoue, Shintaro Inoue Innovative Beauty Science Laboratory, Kanebo Cosmetics Inc., Odawara, JapanSearch for more papers by this authorYoshiki Miyachi, Yoshiki Miyachi Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, JapanSearch for more papers by this author Mariko Hara-Chikuma, Corresponding Author Mariko Hara-Chikuma [email protected] Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, Japan Innovative Beauty Science Laboratory, Kanebo Cosmetics Inc., Odawara, JapanCorrespondence: Center for Innovation in Immunoregulative Technology and Therapeutics, Graduate School of Medicine, Kyoto University, Yoshida Konoe-cho, Sakyo-ku, Kyoto 606-8501, Japan. E-mail: [email protected]Search for more papers by this authorYoshinori Sugiyama, Yoshinori Sugiyama Innovative Beauty Science Laboratory, Kanebo Cosmetics Inc., Odawara, JapanSearch for more papers by this authorKenji Kabashima, Kenji Kabashima Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, JapanSearch for more papers by this authorEisei Sohara, Eisei Sohara Department of Nephrology, Tokyo Medical and Dental University, Tokyo, JapanSearch for more papers by this authorShinichi Uchida, Shinichi Uchida Department of Nephrology, Tokyo Medical and Dental University, Tokyo, JapanSearch for more papers by this authorSei Sasaki, Sei Sasaki Department of Nephrology, Tokyo Medical and Dental University, Tokyo, JapanSearch for more papers by this authorShintaro Inoue, Shintaro Inoue Innovative Beauty Science Laboratory, Kanebo Cosmetics Inc., Odawara, JapanSearch for more papers by this authorYoshiki Miyachi, Yoshiki Miyachi Department of Dermatology, Graduate School of Medicine, Kyoto University, Kyoto, JapanSearch for more papers by this author First published: 03 October 2011 https://doi.org/10.1096/fj.11-186627Citations: 1 This article includes supplemental data. Please visit http://www.fasebj.org to obtain this information. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL ABSTRACT Dendritic cells (DCs) have the ability to present antigen and play a critical role in the induction of the acquired immune response. Skin DCs uptake antigen and subsequently migrate to regional draining lymph nodes (LNs), where they activate naive T cells. Here we show that the water/glycerol channel protein aquaporin 7 (AQP7) is expressed on epidermal and dermal DCs and involved in the initiation of primary immune responses. AQP7-deficient DCs showed a decreased cellular uptake of low-molecular-mass compounds (fluorescein isothiocyanate and Lucifer yellow) and high-molecular-mass substances (ovalbumin and dextran), suggesting that AQP7 is involved in antigen uptake. AQP7-deficient DCs also exhibited reduced chemokine-dependent cell migration in comparison to wild-type DCs. Consistent with these in vitro results, AQP7-deficient mice demonstrated a reduced accumulation of antigen-retaining DCs in the LNs after antigen application to the skin, which could be attributed to decreased antigen uptake and migration. Coincidentally, AQP7-deficient mice had impaired antigen-induced sensitization in a contact hypersensitivity model. These observations suggested that AQP7 in skin DCs is primarily involved in antigen uptake and in the subsequent migration of DCs and is responsible for antigen presentation and the promotion of downstream immune responses.—Hara-Chikuma, M., Sugiyama, Y., Kabashima, K., Sohara, E., Uchida, S., Sasaki, S., Inoue, S., Miyachi, Y. Involvement of aquaporin-7 in the cutaneous primary immune response through modulation of antigen uptake and migration in dendritic cells. FASEB J. 26, 211–218 (2012). www.fasebj.org Citing Literature Supporting Information Filename Description fsb2fj11186627-sup-0001-S1.pdf158 KB Supporting Information Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume26, Issue1January 2012Pages 211-218 RelatedInformation