Portal de Periódicos da CAPES

Generation of Alveolar Epithelial Spheroids via Isolated Progenitor Cells from Human Pluripotent Stem Cells

2014; Elsevier BV; Volume: 3; Issue: 3 Linguagem: Inglês

10.1016/j.stemcr.2014.07.005

2213-6711

Shimpei Gotoh, Isao Ito, Tadao Nagasaki, Yuki Yamamoto, Satoshi Konishi, Y. Korogi, Hisako Matsumoto, Shigeo Muro, Toyohiro Hirai, Michinori Funato, Shin-Ichi Mae, Tarō Toyoda, Aiko Sato‐Otsubo, Seishi Ogawa, Kenji Osafune, Michiaki Mishima,

Congenital heart defects research

No methods for isolating induced alveolar epithelial progenitor cells (AEPCs) from human embryonic stem cells (hESCs) and induced pluripotent stem cells (hiPSCs) have been reported.Based on a study of the stepwise induction of alveolar epithelial cells (AECs), we identified carboxypeptidase M (CPM) as a surface marker of NKX2-1 + ''ventralized'' anterior foregut endoderm cells (VAFECs) in vitro and in fetal human and murine lungs.Using SFTPC-GFP reporter hPSCs and a 3D coculture system with fetal human lung fibroblasts, we showed that CPM + cells isolated from VAFECs differentiate into AECs, demonstrating that CPM is a marker of AEPCs.Moreover, 3D coculture differentiation of CPM + cells formed spheroids with lamellar-body-like structures and an increased expression of surfactant proteins compared with 2D differentiation.Methods to induce and isolate AEPCs using CPM and consequently generate alveolar epithelial spheroids would aid human pulmonary disease modeling and regenerative medicine.