Produção Nacional
Revisado por pares
Brazilian coffee genome project: an EST-based genomic resource
2006; SciELO; Volume: 18; Issue: 1 Linguagem: Inglês
10.1590/s1677-04202006000100008
ISSN1677-9452
AutoresLuiz Gonzaga Esteves Vieira, Alan Carvalho Andrade, Carlos Augusto Colombo, Ana Heloneida de Araújo Moraes, Ângela Metha, Angélica Carvalho de Oliveira, Carlos Alberto Labate, Celso Luís Marino, Cláudia Barros Monteiro-Vitorello, Damares de Castro Monte, Éder Antônio Giglioti, Edna Teruko Kimura, Eduardo Romano, Eiko E. Kuramae, Eliana Gertrudes de Macedo Lemos, Elionor Rita Pereira de Almeida, Érika Cristina Jorge, Érika V. S. Albuquerque, Felipe Rodrigues da Silva, Felipe Vinecky, Haiko Enok Sawazaki, Hamza Fahmi Ali El Dorry, Helaine Carrer, Ilka N. Abreu, João A. N. Batista, João Batista Teixeira, João Paulo Kitajima, Karem Guimarães Xavier, Liziane Maria de Lima, Luís Eduardo Aranha Camargo, Luiz Filipe Protásio Pereira, Luiz Lehmann Coutinho, Manoel Victor Franco Lemos, M. R. Romano, Marcos Antônio Machado, Marcos Mota do Carmo Costa, Maria Fátima Grossi‐de‐Sá, Maria Helena S. Goldman, Maria Inês Tiraboschi Ferro, M. L. P. Tinoco, Mariana C. Oliveira, Marie‐Anne Van Sluys, Milton Massao Shimizu, Mirian Perez Maluf, M. T. S. Eira, Oliveiro Guerreiro Filho, Paulo Arruda, Paulo Mazzafera, Pilar Mariani, Regina L.B.C. de Oliveira, Ricardo Harakava, Silvia Filippi Balbao, Siu Mui Tsai, Sônia Marli Zingaretti, Suzana Neiva Santos, Walter José Siqueira, Gustavo G.L. Costa, Eduardo Fernandes Formighieri, Marcelo Falsarella Carazzolle, Gonçalo Amarante Guimarães Pereira,
Tópico(s)Plant Virus Research Studies
ResumoCoffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa, representing specific stages of cells and plant development that after trimming resulted in 130,792, 12,381 and 10,566 sequences for each species, respectively. The ESTs clustered into 17,982 clusters and 32,155 singletons. Blast analysis of these sequences revealed that 22 % had no significant matches to sequences in the National Center for Biotechnology Information database (of known or unknown function). The generated coffee EST database resulted in the identification of close to 33,000 different unigenes. Annotated sequencing results have been stored in an online database at <A HREF="http://www.lge.ibi.unicamp.br/cafe">http://www.lge.ibi.unicamp.br/cafe</A>. Resources developed in this project provide genetic and genomic tools that may hold the key to the sustainability, competitiveness and future viability of the coffee industry in local and international markets.
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