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Establishment of a continuous tumor‐cell line (PANC‐1) from a human carcinoma of the exocrine pancreas

1975; Wiley; Volume: 15; Issue: 5 Linguagem: Inglês

10.1002/ijc.2910150505

1097-0215

Michael M. Lieber, Joann Mazzetta, Walter A. Nelson‐Rees, Michael J. Kaplan, George J. Todaro,

Pancreatic and Hepatic Oncology Research

An epithelioid cell line, started from a human pancreatic carcinoma of ductal cell origin, has been maintained in culture for over 2 years and has been subcultured more than 40 times. The PANC-1 cell line has a doubling time of 52 h and G6PD activity of the slow mobility of B type. Chromosome studies show a modal number of 63 with three distinct marker chromosomes and a small ring chromosome. The malignant nature of the PANC-1 cell line was verified by: (1) the ready growth of PANC-1 cells in soft agar and on top of a fibroblast monolayer; and (2) the formation of a progressively growing anaplastic carcinoma after injection of a nude-athymic mouse with PANC-1 cells.