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BIBTEX RIS

Characterization of Class I Newcastle Disease Virus Isolates from Hong Kong Live Bird Markets and Detection Using Real-Time Reverse Transcription-PCR

2007; American Society for Microbiology; Volume: 45; Issue: 4 Linguagem: Inglês

10.1128/jcm.02594-06

ISSN

1098-660X

Autores

L. Mia Kim, Daniel J. King, David L. Suarez, Chun Wai Wong, Claudio L. Afonso,

Tópico(s)

Viral Infections and Vectors

Resumo

Newcastle disease viruses isolated from Hong Kong live bird markets (LBMs) were not detected by a USDA-validated matrix gene real-time reverse transcription-PCR (RT-PCR) assay. Based upon phylogenetic analysis of the fusion gene, these viruses were related to lentogenic class I viruses found in U.S. LBMs and wild waterfowl. An alternative real-time RT-PCR assay which complements the matrix gene assay was developed to efficiently detect class I viruses.

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