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Improving analysis from second-derivative uv-absorption spectrometry

1978; Optica Publishing Group; Volume: 17; Issue: 5 Linguagem: Inglês

10.1364/ao.17.000724

0003-6935

A.R. Hawthorne, J.H. Thorngate,

Spectroscopy and Chemometric Analyses

Methods for improving the analytical results obtained from second-derivative spectrometry are presented. By using a combination of the first and second derivatives of the spectral data, a response varying linearly with concentration can be obtained at all wavelengths if Beer's law applies. A least-squares analysis of the data using standard spectra can then be utilized. Special emphasis is given to improving the response from a wavelength-modulated derivative uv-absorption spectrometer. Simulated examples are provided showing the improved analytical capabilities using the procedure outlined.