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Overview of Affinity Tags for Protein Purification

2004; Wiley; Volume: 36; Issue: 1 Linguagem: Inglês

10.1002/0471140864.ps0909s36

1934-3663

Michelle E. Kimple, John Sondek,

Biochemical and Structural Characterization

Abstract Addition of an affinity tag is a useful method for differentiating recombinant proteins expressed in bacterial and eukaryotic expression systems from the background of total cellular proteins, and for detecting protein‐protein interactions. This overview describes the historical basis for the development of affinity tags, affinity tags that are commonly used today, how to choose an appropriate affinity tag for a particular purpose, and several recently developed affinity tag technologies that may prove useful in the near future.