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Reemergence of dengue virus type-3 (subtype-III) in India: Implications for increased incidence of DHF & DSS

2006; BioMed Central; Volume: 3; Issue: 1 Linguagem: Inglês

10.1186/1743-422x-3-55

1743-422X

Paban Kumar Dash, Man Mohan Parida, Parag Saxena, Ajay Abhyankar, CP Singh, KN Tewari, Asha Mukul Jana, K. Sekhar, PV Lakshmana Rao,

Malaria Research and Control

Abstract Background Dengue virus infection has recently taken endemic proportion in India implicating all the four known dengue serotypes. There was a major dengue outbreak in northern India including Delhi in October- December, 2003 and again in 2004. We have carried out a detailed investigation of the 2004 outbreak by Serosurveillance, RT-PCR, nested PCR, virus isolation and genotyping. We also report the molecular epidemiological investigation of these outbreaks. Results The serological investigation of 162 suspected serum samples using an in-house dengue dipstick ELISA revealed 11%-IgM, 51%-IgG and 38%-both IgM and IgG antibody positivity. The RT-PCR analysis revealed presence of dengue RNA in 17 samples. Further subtyping and genotyping by nested PCR and nucleotide sequencing of C-prM gene junction revealed the association of subtype III of dengue virus type 3 in the outbreak. Conclusion The sudden shifting and dominance of the dengue virus serotype-3 (subtype III) replacing the earlier circulating serotype-2 (subtype IV) is a point of major concern and may be attributed to increased incidence of DHF and DSS in India.