Diagnosis of Methylmalonic Acidemia from Dried Blood Spots by HPLC and Intramolecular-Excimer Fluorescence Derivatization
2004; American Association for Clinical Chemistry; Volume: 51; Issue: 1 Linguagem: Inglês
10.1373/clinchem.2004.040022
ISSN1530-8561
AutoresOsama Y. Al-Dirbashi, Minnie Jacob, Zuhair N. Al‐Hassnan, Fahad El-Badaoui, Mohamed S. Rashed,
Tópico(s)Amino Acid Enzymes and Metabolism
Resumoregarded as very specific (1, 6 ).Because of this specificity, a proportion of patients who are investigated for vitamin B 12 deficiency will end up being tested for the presence of IFABs, regardless of the diagnostic cascade favored by the investigating physician(s) (1, 2 ).In referral laboratories, this may lead to substantial test volumes of labor-intensive manual RIAs, with all the associated problems.These range from an increased risk of sample mix-ups or analytical mishaps attributable to multiple manual samplehandling steps, to difficulties in maintaining consistent performance, to the need to handle and dispose of radioactivity, and to lower analytical precision and increased turnaround time compared with automated assays.The new automated IFAB assay addresses all of these problems while maintaining comparable diagnostic accuracy.Moreover, both vitamin B 12 measurements and IFAB measurements can be performed on the same instrument, with the possibility to tag IFAB testing to individual vitamin B 12 samples, conditional on the measured cobalamin concentration, a further improvement in workflow.We therefore believe that the availability of this new IFAB assay provides an opportunity for increased and more expeditious testing and diagnosis of pernicious anemia.The Mayo Foundation received funds from Beckman Coulter for the validation of this IF antibody assay.
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