Produção Nacional
Hypercholesterolemic diet induces hepatic steatosis and alterations in mRNA expression of NADPH oxidase in rat livers
2014; Editora da Universidade de São Paulo; Volume: 58; Issue: 3 Linguagem: Inglês
10.1590/0004-2730000002831
ISSN1677-9487
AutoresIsabel Cristina Mallosto Emerich de Abreu, Joyce Ferreira da Costa Guerra, Renata Rebeca Pereira, Maísa Silva, Wanderson Geraldo de Lima, Marcelo Eustáquio Silva, Maria Lúcia Pedrosa,
Tópico(s)Pancreatic function and diabetes
ResumoObjective : This study aimed to determine whether a hypercholesterolemic diet induces hepatic steatosis, alterations in mRNA expression of NADPH oxidase subunits, and antioxidant defenses.Materials and methods : Fischer rats were divided into two groups of eight animals according to the treatment, control (C) and hypercholesterolemic diet (H). Those in group C were fed a standard diet (AIN-93M), and those of the group H were fed a hypercholesterolemic diet (25% soybean oil and 1% cholesterol).Results : The hypercholesterolemic diet did not affect body weight, but resulted in the accumulation of lipids in the liver, increased serum activities of aminotransferases and cholesterol levels. Biomarker of lipid peroxidation (TBARS) and mRNA expression of NADPH oxidase subunits p22phox and p47phox were increased in the liver of animals in group H. Besides, the activity and expression of antioxidant enzymes were altered.Conclusion : The results show increased mRNA expression of NADPH oxidase subunits and changes in antioxidant enzyme activities in diet-induced hepatic steatosis. Arq Bras Endocrinol Metab. 2014;58(3):251-9
Referência(s)