Short-Sequence Tandem and Nontandem DNA Repeats and Endogenous Hydrogen Peroxide Production Contribute to Genetic Instability of Streptococcus pneumoniae
2002; American Society for Microbiology; Volume: 184; Issue: 16 Linguagem: Inglês
10.1128/jb.184.16.4392-4399.2002
ISSN1098-5530
AutoresChristopher D. Pericone, Deborah Bae, Mikhail Shchepetov, Tera L. McCool, Jeffrey N. Weiser,
Tópico(s)Cystic Fibrosis Research Advances
ResumoABSTRACT Loss-of-function mutations in the following seven pneumococcal genes were detected and analyzed: pspA , spxB , xba , licD2 , lytA , nanA , and atpC . Factors associated with these mutations included (i) frameshifts caused by reversible gain and loss of single bases within homopolymeric repeats as short as 6 bases, (ii) deletions caused by recombinational events between nontandem direct repeats as short as 8 bases, and (iii) substitutions of guanine residues caused at an increased frequency by the high levels of hydrogen peroxide (>2 mM) typically generated by this species under aerobic growth conditions. The latter accounted for a frequency as high as 2.8 × 10 −6 for spontaneous mutation to resistance to optochin and was 10- to 200-fold lower in the absence of detectable levels of H 2 O 2 . Some of these mutations appear to have been selected for in vivo during pneumococcal infection, perhaps as a consequence of immune pressure or oxidative stress.
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