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Analysis of HPV-other Samples by Performing HPV DNA Sequencing

2009; Korean Society of Pathologists & the Korean Society for Cytopathology; Volume: 43; Issue: 3 Linguagem: Inglês

10.4132/koreanjpathol.2009.43.3.250

2092-8920

Yoo Duk Choi, Changwoo Han, Woon-Jae Chung, Woon‐Won Jung, Ji Shin Lee, Jong‐Hee Nam, Min Cheol Lee, Sang‐Woo Juhng, Ho-Sun Choi, Chang-Soo Park,

Molecular Biology Techniques and Applications

BACKGROUNDHPV-other samples are designated as being positive on HPV-PCR, but negative when using specific HPV hybridization probes. We wanted to determine the types on the HPV-other samples by performing sequencing, and to know the pathologic status of the uterine cervix according to the HPV type detected on sequencing.METHODSFor HPV genotying, we used the commercially available HPV DNA Chip test, which contains 15 types of high-risk HPV and 9 types of low-risk HPV. The HPV DNA sequencing was performed for the HPV-other samples of 209 patients who subsequently underwent cervical biopsy.RESULTSFor 204 of the 209 samples, the HPV types detected by sequencing were absent types at used HPV DNA chip. For the remaining 5 samples, sequencing was impossible due to mixed peaks. HPV-81 (19.6%), HPV-61 (18.6%), HPV-62 (16.7%) and HPV-84 (13.9%) were frequently detected. For the HPV-81, -62, -71, and -72 samples, most of the samples displayed normal or LSIL. However, HPV-84 and -61 were more associated with HSIL or worse, as compared to the other types.ConclusionHPV-81, -61, -62 and -84 were frequently found on sequencing analysis of the HPV-other samples. The pathologic status was diverse, according to the HPV type detected on sequencing.