Produção Nacional
Revisado por pares
Relationship between the epithelial expression of h MLH 1, MDM 2, and p63 and lower lip carcinogenesis
2013; Wiley; Volume: 43; Issue: 5 Linguagem: Inglês
10.1111/jop.12144
ISSN1600-0714
AutoresDenise Hélen Imaculada Pereira de Oliveira, Maria Luiza Diniz de Sousa Lopes, Dmitry José de Santana Sarmento, Lélia María Guedes Queiroz, Márcia Cristina da Costa Miguel, Éricka Janine Dantas da Silveira,
Tópico(s)Salivary Gland Tumors Diagnosis and Treatment
ResumoObjectives To evaluate the relationship between the epithelial expression of h MLH 1, MDM 2, and p63 in lower lip carcinogenesis, comparing the immunostaining of these proteins in cases of actinic cheilitis ( AC ) and lower lip squamous cell carcinoma ( SCC ). Study Design Forty cases of AC and 40 cases of SCC were studied, both lesions were of lower lip. Histological sections of 3 μm were submitted to immunoperoxidase method, and 1000 cells were counted for immunohistochemical analysis of lesions. The results were analyzed quantitatively, and expression was compared by the M ann– W hitney, S tudent t ‐test, or one‐way ANOVA , adopting a level of significance of 5%. Results A higher percentage of epithelial cells expressing h MLH 1 was observed in cases of AC without dysplasia or mild dysplasia (721.23 ± 88.116), whereas fewer positive cells were observed in lower lip SSC s (255.03 ± 199.47) when compared to the AC group ( P < 0.001). Immunoexpression of MDM 2 was higher in SCC s of the lower lip compared with AC ( P = 0.019). For p63 protein, the expression was higher in AC than in SCC ( P = 0.045). Conclusion The present results showed changes in the immunoexpression of h MLH 1, MDM 2, and p63 in epithelial cells from premalignant and malignant lip disease, supporting the hypothesis that these alterations are related to the process of lower lip carcinogenesis.
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