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Biological effects of 6 mT static magnetic fields: A comparative study in different cell types

2006; Wiley; Volume: 27; Issue: 7 Linguagem: Inglês

10.1002/bem.20252

1521-186X

Bernadette Tenuzzo, Alfonsina Chionna, Elisa Panzarini, Remigio Lanubile, Patrizia Tarantino, Bruno Di Jeso, Majdi Dwikat, Luciana Dini,

Magnetic and Electromagnetic Effects

BioelectromagneticsVolume 27, Issue 7 p. 560-577 Article Biological effects of 6 mT static magnetic fields: A comparative study in different cell types† Bernadette Tenuzzo, Bernadette Tenuzzo Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorAlfonsina Chionna, Alfonsina Chionna Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorElisa Panzarini, Elisa Panzarini Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorRemigio Lanubile, Remigio Lanubile Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorPatrizia Tarantino, Patrizia Tarantino Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorBruno Di Jeso, Bruno Di Jeso Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorMajdi Dwikat, Majdi Dwikat Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorLuciana Dini, Corresponding Author Luciana Dini [email protected] Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalyDepartment of Biological and Environmental Science and Technology, University of Lecce, Via per Monteroni 73100 Lecce, Italy.Search for more papers by this author Bernadette Tenuzzo, Bernadette Tenuzzo Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorAlfonsina Chionna, Alfonsina Chionna Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorElisa Panzarini, Elisa Panzarini Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorRemigio Lanubile, Remigio Lanubile Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorPatrizia Tarantino, Patrizia Tarantino Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorBruno Di Jeso, Bruno Di Jeso Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorMajdi Dwikat, Majdi Dwikat Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalySearch for more papers by this authorLuciana Dini, Corresponding Author Luciana Dini [email protected] Department of Biological and Environmental Science and Technology, University of Lecce, Lecce, ItalyDepartment of Biological and Environmental Science and Technology, University of Lecce, Via per Monteroni 73100 Lecce, Italy.Search for more papers by this author First published: 24 May 2006 https://doi.org/10.1002/bem.20252Citations: 71 † This work is dedicated to the memory of Luigi Abbro. AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract The present work was a comparative study of the bio-effects induced by exposure to 6 mT static magnetic field (MF) on several primary cultures and cell lines. Particular attention was dedicated to apoptosis. Cell viability, proliferation, intracellular Ca2+ concentration and morphology were also examined. Primary cultures of human lymphocytes, mice thymocytes and cultures of 3DO, U937, HeLa, HepG2 and FRTL-5 cells were grown in the presence of 6 mT static MF and different apoptosis-inducing agents (cycloheximide, H2O2, puromycin, heat shock, etoposide). Biological effects of static MF exposure were found in all the different cells examined. They were cell type-dependent but apoptotic inducer-independent. A common effect of the exposure to static MF was the promotion of apoptosis and mitosis, but not of necrosis or modifications of the cell shape. Increase of the intracellular levels of Ca2+ ions were also observed. When pro-apoptotic drugs were combined with static MF, the majority of cell types rescued from apoptosis. To the contrary, apoptosis of 3DO cells was significantly increased under simultaneous exposure to static MF and incubation with pro-apoptotic drugs. From these data we conclude that 6 mT static MF exposure interfered with apoptosis in a cell type- and exposure time-dependent manner, while the effects of static MF exposure on the apoptotic program were independent of the drugs used. Bioelectromagnetics 27:560–577, 2006. © 2006 Wiley-Liss, Inc. Citing Literature Volume27, Issue7October 2006Pages 560-577 RelatedInformation