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Mapping protein-specific micro-environments in live cells by fluorescence lifetime imaging of a hybrid genetic-chemical molecular rotor tag

2012; Royal Society of Chemistry; Volume: 48; Issue: 69 Linguagem: Inglês

10.1039/c2cc33133k

1364-548X

Evangelos Gatzogiannis, Zhixing Chen, Lu Wei, Richard Wombacher, Ya‐Ting Kao, Grygorii Yefremov, Virginia W. Cornish, Wei Min,

Lipid Membrane Structure and Behavior

The micro-viscosity and molecular crowding experienced by specific proteins can regulate their dynamics and function within live cells. Taking advantage of the emerging TMP-tag technology, we present the design, synthesis and application of a hybrid genetic-chemical molecular rotor probe whose fluorescence lifetime can report protein-specific micro-environments in live cells.