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Retention of sperm motility in turbot, Scophthalmus maximus L.: the effects of time from activation, thermal shock and adenosine triphosphate levels

1993; Wiley; Volume: 24; Issue: 2 Linguagem: Inglês

10.1111/j.1365-2109.1993.tb00542.x

1365-2109

Audrey J. Geffen, O. FRAYER,

Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities

Aquaculture ResearchVolume 24, Issue 2 p. 203-209 Retention of sperm motility in turbot, Scophthalmus maximus L.: the effects of time from activation, thermal shock and adenosine triphosphate levels A. J. GEFFEN, Corresponding Author A. J. GEFFEN University of Liverpool, Port Erin Marine Laboratory, Port Erin, Isle of Man, UKDr A.J. Geffen, University of Liverpool, Port Erin Marine Laboratory, Port Erin, Isle of Man, UK.Search for more papers by this authorO. FRAYER, O. FRAYER Institut Supérieur Agricole de Beauvais, Beauvais, FranceSearch for more papers by this author A. J. GEFFEN, Corresponding Author A. J. GEFFEN University of Liverpool, Port Erin Marine Laboratory, Port Erin, Isle of Man, UKDr A.J. Geffen, University of Liverpool, Port Erin Marine Laboratory, Port Erin, Isle of Man, UK.Search for more papers by this authorO. FRAYER, O. FRAYER Institut Supérieur Agricole de Beauvais, Beauvais, FranceSearch for more papers by this author First published: March 1993 https://doi.org/10.1111/j.1365-2109.1993.tb00542.xCitations: 8AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Abstract Four parameters were examined in order to define sperm quality in turbot Scophthalmus maximus L., sperm: (1) sperm motility, measured by direct counts of the number of active spermatozoa, expressed as % of total spermatozoa; (2) retention of motility after activation, measured by direct counts, 0–60min after activation, expressed as a % of the initial level of activity; (3) resistance to thermal stress, measured as change in retention of motility, and (4) adenosine phosphate (ATP) concentration, determined for samples of non-activated sperm. The proportion of motile spermatozoa at activation ranged from 34·8% to 97·6% (mean 76·3%) for the individual males tested. Turbot sperm retained on average 52% (range 27–90%) of its initial activity one hour after activation. Sperm samples which were stressed by cooling to –27°C retained only 8·6% (range 0–25%) of initial activity, compared to control samples which retained 49% (range 38–63%) of initial activity. The retention of motility after activation was not significantly related to the initial motility or the levels of ATP. Concentrations of ATP in turbot sperm (mean 0·46mg ATP/106 spermatozoa, equivalent to 9·2nmol ATP/108 spermatozoa) were comparable to those measured in mammals. Citing Literature Volume24, Issue2March 1993Pages 203-209 RelatedInformation