Ethanol modulates apolipoprotein B mRNA editing in the rat.
1996; Elsevier BV; Volume: 36; Issue: 10 Linguagem: Inglês
10.1016/s0022-2275(20)39192-6
ISSN1539-7262
AutoresPaul P. Lau, Dolores J. Cahill, Hengyue Zhu, Lawrence Chan,
Tópico(s)RNA modifications and cancer
ResumoWe have studied the rat ethanol-liquid diet model for chronic ethanol modulation of lipid homeostasis, apolipoprotein (apo) B production and apoB mRNA editing.Male Wistar rats were fed one of three diets: i) regular chow, ii) an isocaloric liquid diet, or iii) isocaloric ethanol-liquid diet where ethanol accounts for 35.5% of the total calories, for up to 40 days.There was no difference in body weight or liver/body weight ratio among the three groups of animals at the end of the feeding period.Hepatic and plasma triglycerides were elevated in the ethanol-treated animals only, correlated with an accumulation of lipid particles in the liver of these animals.By DNA excess hybridization, the steady state mRNA levels of apoB and apoB mRNAediting protein relative to actin were not significantly altered.The proportion of edited apoB mRN& Le., apoB-48 mRNA/(apoB-48 + E 100) mRNA, increased in a timedependent manner from -50% to 100% in the ethanol-treated group.It remained unchanged in the chow-and liquid diet-fed animals.The proportion of apoB48/apoE100 protein synthesis was determined by [%]methionine labeling followed by specific immunoprecipitation and SDS-polyacrylamide gel electrophoresis.The amount of newly synthesized apoB48 increased from Supplementary key words apoBlOO apoB48 plasma triglycerides triglyceride metabolism Abbreviations: apo, apolipoprotein; FPLC, fast protein liquid chromatography; VLDL, very low density lipoprotein; LDL, low density lipoprotein; HDL, high density lipoprotein; RT-PCR, reverse transcriptase-polymerase chain reaction; TCA, trichloroacetic acid; ApoB mRNA editing is a posttranscriptionally regu-REP^, protein.Iated process that is coincident with splicing and 'To whom correspondence should be addressed.
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