Cell-Free Fetal DNA Is Not Present in Plasma of Nonpregnant Mothers
2004; American Association for Clinical Chemistry; Volume: 50; Issue: 3 Linguagem: Inglês
10.1373/clinchem.2003.023358
ISSN1530-8561
AutoresRobbert J. P. Rijnders, G. C. M. L. Christiaens, Aïcha Ait Soussan, C. Ellen van der Schoot,
Tópico(s)Parvovirus B19 Infection Studies
ResumoFetal DNA sequences are present in the plasma of pregnant women (1) and can be studied to determine fetal sex(1) and RhD type (2). Unlike fetal cells, fetal cell-free DNA is cleared rapidly from plasma after delivery (3), with a half-life of 16–28 min. Even when clearance is slowed, as in preeclampsia (4), the mean half-life for clearance of fetal DNA is 114 min (5). Recently, Invernizzi et al. (6) reported positive PCR results for a Y-chromosome-specific sequence in 36 of 160 (22%) women who had given birth to a son up to several years previously. By contrast, other published studies on pregnant women have described no false-positive results (1)(2)(7)(8)(9)(10)(11)(12)(13)(14). It is conceivable that fetal cells remain and proliferate in the maternal circulation or are engrafted in maternal organs after delivery and that this proliferation is suppressed again in a subsequent pregnancy. Lambert et al. (15) also recently reported the presence of fetal DNA in plasma of nonpregnant women. They also found male DNA sequences in 8 of 22 (36%) healthy nonpregnant women who had previously given birth to sons, but filtration studies showed that this DNA was not cell free. We recruited 120 nonpregnant women who, with their consent, donated 10 mL of blood. We recorded the numbers and sexes of their children and numbers of abortions and extra-uterine pregnancies. Blood was collected in an EDTA-containing Vacutainer Tube and sent to the Central Laboratory of the Dutch Red Cross in Amsterdam, where samples were processed within 24 h. Each sample was centrifuged at 1289 g for 10 min. The supernatant was collected and centrifuged again at 2773 g for 20 min and stored at −30 … [↵][1]aAddress correspondence to this author at: Division of Internal Medicine, Department of Medicine, Surgery and Dentistry, San Paolo School of Medicine, Via di Rudini 8, 20142 Milan, Italy. Fax 39-02-50323089; e-mail pietro.invernizzi{at}unimi.it. [1]: #xref-corresp-2-1
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