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Direct Allelic Variation Scanning of the Yeast Genome

1998; American Association for the Advancement of Science; Volume: 281; Issue: 5380 Linguagem: Inglês

10.1126/science.281.5380.1194

1095-9203

Elizabeth A. Winzeler, Daniel R. Richards, Andrew Conway, Alan L. Goldstein, Sue Kalman, Michael McCullough, John H. McCusker, David A. Stevens, Lisa Wodicka, David J. Lockhart, Ronald W. Davis,

Plant Disease Resistance and Genetics

As more genomes are sequenced, the identification and characterization of the causes of heritable variation within a species will be increasingly important. It is demonstrated that allelic variation in any two isolates of a species can be scanned, mapped, and scored directly and efficiently without allele-specific polymerase chain reaction, without creating new strains or constructs, and without knowing the specific nature of the variation. A total of 3714 biallelic markers, spaced about every 3.5 kilobases, were identified by analyzing the patterns obtained when total genomic DNA from two different strains of yeast was hybridized to high-density oligonucleotide arrays. The markers were then used to simultaneously map a multidrug-resistance locus and four other loci with high resolution (11 to 64 kilobases).