Portal de Periódicos da CAPES

Aequorea victoria bioluminescence moves into an exciting new era

1998; Elsevier BV; Volume: 16; Issue: 5 Linguagem: Inglês

10.1016/s0167-7799(98)01184-6

0167-9430

Jonathan M. Kendall, Michael N. Badminton,

Advanced Fluorescence Microscopy Techniques

Bioluminescence has revolutionized research into many cellular and molecular-biological processes, ranging from intracellular signalling to gene transcription. This article focuses on the chemistry and biotechnological exploitation of the two proteins involved in bioluminescence of the jellyfish Aequorea victoria—aequorin and green fluorescent protein. Engineered recombinant aequorin has led to a novel technological approach to monitoring calcium signals in organelles and subcellular domains. A new generation of intracellular calcium indicators has been produced in which engineered variants of green fluorescent protein are used to probe their ionic environment using intramolecular fluorescence-resonance-energy transfer.