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Molecular characterization of DDX26, a human DEAD-box RNA helicase, located on chromosome 7p12

2001; Associação Brasileira de Divulgação Científica; Volume: 34; Issue: 10 Linguagem: Inglês

10.1590/s0100-879x2001001000002

1414-431X

Anamaria A. Camargo, D. N. Nunes, Helena B. Samaia, L. Liu, V. Peter Collins, Andrew J.G. Simpson, Emmanuel Dias‐Neto,

RNA and protein synthesis mechanisms

DEAD-box proteins comprise a family of ATP-dependent RNA helicases involved in several aspects of RNA metabolism. Here we report the characterization of the human DEAD-box RNA helicase DDX26. The gene is composed of 14 exons distributed over an extension of 8,123 bp of genomic sequence and encodes a transcript of 1.8 kb that is expressed in all tissues evaluated. The predicted amino acid sequence shows a high similarity to a yeast DEAD-box RNA helicase (Dbp9b) involved in ribosome biogenesis. The new helicase maps to 7p12, a region of frequent chromosome amplifications in glioblastomas involving the epidermal growth factor receptor (EGFR) gene. Nevertheless, co-amplification of DDX26 with EGFR was not detected in nine tumors analyzed.