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Convergent Chemical Synthesis of [Lysine 24, 38, 83 ] Human Erythropoietin

2011; Wiley; Volume: 51; Issue: 4 Linguagem: Inglês

10.1002/anie.201106060

1521-3773

Suhuai Liu, Brad L. Pentelute, Stephen B. H. Kent,

Computational Drug Discovery Methods

A new route to EPO: Nonglycosylated human erythropoietin (EPO) was prepared using a convergent chemical ligation synthetic strategy. The synthetic [Lysine24, 38, 83] EPO analogue, which was purified by HPLC and has the correct molecular weight (see picture), shows well-defined covalent structure, is correctly folded, and is biologically active in a factor-dependent cell line assay. Detailed facts of importance to specialist readers are published as "Supporting Information". Such documents are peer-reviewed, but not copy-edited or typeset. They are made available as submitted by the authors. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.