Development and Validation of a Sensitive and Specific sodB -Based Quantitative PCR Assay for Molecular Detection of Ehrlichia Species
2014; American Society for Microbiology; Volume: 52; Issue: 11 Linguagem: Inglês
10.1128/jcm.02340-14
ISSN1098-660X
AutoresBarbara A. Qurollo, Dana Riggins, Alaire Comyn, Merone T. Zewde, Edward B. Breitschwerdt,
Tópico(s)Antimicrobial Peptides and Activities
ResumoWe developed a sensitive and specific sodB-based quantitative PCR assay to detect Ehrlichia spp. The assay's limit of detection was 5 copies/reaction, and it did not amplify nonspecific DNA. Compared with a 16S rRNA gene PCR target, the sodB target may offer an improved molecular diagnostic assay to detect Ehrlichia spp.
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