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PD-1 deficiency results in the development of fatal myocarditis in MRL mice

2010; Oxford University Press; Volume: 22; Issue: 6 Linguagem: Inglês

10.1093/intimm/dxq026

1460-2377

Jian Wang, Il‐mi Okazaki, Taku Yoshida, Shunsuke Chikuma, Yu Kato, Fumio Nakaki, Hiroshi Hiai, Tasuku Honjo, Taku Okazaki,

T-cell and B-cell Immunology

The deficiency of programmed cell death 1 (PD-1, Pdcd1), a negative immuno-receptor belonging to the CD28/cytotoxic T lymphocyte antigen 4 (CTLA-4) family, can support various tissue-specific autoimmune conditions. Here, we analyzed the effect of PD-1 deficiency in MRL mice that is genetically predisposed to systemic autoimmunity. MRL-Pdcd1−/− mice developed a fatal myocarditis, which is reminiscent of CTLA-4-deficient (Ctla4−/−) mice. Massive infiltration of CD4+ and CD8+ T cells and myeloid cells was found in hearts of MRL-Pdcd1−/− mice concomitant with the production of high-titer auto-antibodies against cardiac myosin. In contrast to Ctla4−/− mice in which most of the CD4+ T cells are non-specifically activated and invade various organs, T cells in the heart but not in the spleen and lymph nodes are activated in MRL-Pdcd1−/− mice, suggesting that myocarditis is mediated by antigen-specific autoimmune response. Heart infiltrating myeloid cells strongly suppressed the allogenic response of T cells in vitro, suggesting that these Mac1+Gr1+ myeloid cells are phenotypically similar to myeloid suppressor cells, which can be found in tumor-bearing hosts. These findings unravel the hidden heart-specific autoimmune predisposition of MRL mice and provide MRL-Pdcd1−/− mice as a useful animal model of lymphocytic myocarditis.