Preparation of photo-cross-linked pH-responsive polypeptide nanogels as potential carriers for controlled drug delivery
2011; Royal Society of Chemistry; Volume: 21; Issue: 30 Linguagem: Inglês
10.1039/c1jm10391a
ISSN1364-5501
AutoresJianxun Ding, Xiuli Zhuang, Chunsheng Xiao, Yilong Cheng, Zhao Li, Chaoliang He, Zhaohui Tang, Xuesi Chen,
Tópico(s)Advanced Polymer Synthesis and Characterization
ResumoDiblock and triblock copolymers, including poly(ethylene glycol monomethyl ether)-b-poly(L-glutamic acid-co-γ-cinnamyl-L-glutamate) (mPEG-b-P(LGA/CLG)) and poly(L-glutamic acid-co-γ-cinnamyl-L-glutamate)-b-poly(ethylene glycol)-b-poly(L-glutamic acid-co-γ-cinnamyl-L-glutamate) (P(LGA/CLG)-b-PEG-b-P(LGA/CLG)), were synthesized by ring-opening polymerization (ROP) of γ-benzyl-L-glutamate-N-carboxyanhydride (BLG-NCA) monomer with PEG-based macroinitiator, deprotection of the benzyl groups and subsequent chemical modification with cinnamyl alcohol. The structures of copolymers were confirmed by 1H NMR and GPC analyses. Pyrene-probe-based fluorescence technique revealed that these diblock and triblock copolymers could self-assemble into micelles in aqueous solution at pH 7.4 spontaneously, with PEG shells and P(LGA/CLG) cores. Under UV-irradiation at λ = 254 nm, the P(LGA/CLG) blocks in the cores of the micelles were cross-linked through the photodimerization of the cinnamyloxy groups, yielding nanogels. The nanogels were characterized by 1H NMR, FT-IR, SEM, AFM and DLS. The nanogels were pH-responsive and their properties could be tuned by varying the compositions of block copolymers. In vitro MTT assay demonstrated that the nanogels were biocompatible to HeLa cells, rendering their potential for drug delivery applications. Rifampin as a model drug was loaded into the nanogels. The in vitro rifampin release behaviors of nanogels could be affected by both the compositions of block copolymers and solution pH. These properties indicated that the pH-responsive nanogels fabricated by photo-cross-linking polypeptide micelles can be used as drug carriers for intelligent drug delivery.
Referência(s)