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Expression and induction in vitro of macrophage differentiation antigens on murine cell lines.

1983; American Association of Immunologists; Volume: 130; Issue: 1 Linguagem: Inglês

10.4049/jimmunol.130.1.108

1550-6606

P Ralph, M K Ho, P B Litcofsky, Timothy A. Springer,

Biotin and Related Studies

that Mac-1 polypeptides of 95,000 and 170,000 M, and the Mac-2 polypeptide of 32,000 M, were found in lysates of mature macrophage lines but not in other lines, including myeloid or immature leukemias. The Mac-3 antigen was found in large amounts in all macrophage lines and to lesser degrees in some myeloid and B lymphoid lines. The Mr of Mac-3 varied from 100,000 to 170,000, perhaps due to differential glycosylation. Analysis of Mac-1 and Mac-2 antigens by flow cytometry showed expression on all macrophage lines. Similarly, all three Mac antigens were detected in high amounts on macrophage lines by ‘251-labeled antibody binding. Mac-1 and Mac-2 were not routinely seen on other hematopoietic lines, but Mac-3 was expressed in variably low amounts on some lines of myeloid and B cell lineage. These results with macrophage lines and variants exclude a simple relationship between Mac differentiation antigens and certain macrophage characteristics (5’ nucleotidase, cytotoxic capacities, antibody-dependent phagocytosis, and production of oxygen radicals). Mac-1 and Mac-3 but not Mac-2 could be induced in the M1 myeloblast line by corticosteroid, lipopolysaccharide, and several conditioned media containing myeloid colony-stimulating activity. These agents are known to induce a number of other macrophage markers in M1, e.g., Fc and C receptors, phagocytosis, and lysozome production. Therefore, it appears Mac-2 antigen is not required for expression of these characteristics. The levels of Mac-1 and Mac-3 in M1 cells increased slowly over 6 days of stimulation, approaching those found on mature macrophage lines. Although antiMac-1 does not block the detection of Mac-3 antigen on induced M1 cells, the presence of anti-Mac-1 antibody during induction partially blocked the appearance of Mac3 antigen. Thus, the surface structure associated with Mac-1 antigen appears to be involved in differentiation of macrophages.