Detection of cows' milk in ewes' milk and cheese by an indirect enzyme-linked immunosorbent assay (ELISA)
1990; Cambridge University Press; Volume: 57; Issue: 2 Linguagem: Inglês
10.1017/s0022029900026807
ISSN1469-7629
AutoresElena Rodríguez‐Rodríguez, Rosario Martı́n, Teresa Garcı́a, Pablo E. Hernández, Bernabé Sanz,
Tópico(s)Probiotics and Fermented Foods
ResumoSummary An indirect enzyme-linked immunosorbent assay was successfully developed for the detection of defined amounts of cows' milk (1–50%) in sheeps' milk and cheese. The assay used polyclonal antibodies raised in rabbits against bovine caseins (BC). The anti-BC antibodies were recovered from the crude antiserum by immunoadsorption and elution from a column containing BC. The antibodies were biotinylated and rendered cows' milk specific by mixing them with lyophilized ovine and caprine caseins. ExtrAvidin-peroxidase was used to detect the biotinylated anti-BC antibodies bound to BC immobilized on 96-well plates. Subsequent enzymic conversion of substrate gave clear absorbance differences when assaying mixtures of sheeps' milk and cheese containing variable amounts of cows' milk.
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