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Isolation and Structure Determination of Bioactive Isoflavones from Callus Culture of Dipteryx odorata

2005; Pharmaceutical Society of Japan; Volume: 53; Issue: 7 Linguagem: Inglês

10.1248/cpb.53.740

1347-5223

Ana Helena Januário, Miriam Vergínia Lourenço, L.A. Domezio, Rosemeire Cristina Linhari Rodrigues Pietro, Marcelo Santos Castilho, Daniela M. Tomazela, Maria Fátima das Graças Fernandes da Silva, Paulo C. Vieira, João Batista Fernandes, Suzelei de Castro França,

Bioactive natural compounds

Crude extracts of callus and roots of Dipteryx odorata were analyzed by HPLC to detect and quantify isoflavone contents. Based on spectroscopic and X-ray crystallography data the structures of two isoflavones were elucidated as 7-hydroxy-4',6-dimethoxyisoflavone and 3',7-dihydroxy-4',6-dimethoxyisoflavone. The production of dry biomass of 7-hydroxy-4',6-dimethoxyisoflavone in cultured callus was 4.12 mg/g, approximately eleven fold higher than the amount accumulated in roots of D. odorata wild-growing plants. The 7-hydroxy-4',6-dimethoxyisoflavone was effective against glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi. This is the first report on those bioactive isoflavones accumulated in callus of D. odorata.