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Simultaneous determination of metal ions, amino acids, and other small biogenic molecules in human serum by capillary zone electrophoresis with transient isotachophoretic preconcentration

2005; Wiley; Volume: 28; Issue: 6 Linguagem: Inglês

10.1002/jssc.200400070

1615-9314

Hikaru Okamoto, Andrei R. Timerbaev, Takeshi Hirokawa,

Mass Spectrometry Techniques and Applications

Journal of Separation ScienceVolume 28, Issue 6 p. 522-528 Research Article Simultaneous determination of metal ions, amino acids, and other small biogenic molecules in human serum by capillary zone electrophoresis with transient isotachophoretic preconcentration Hikaru Okamoto, Hikaru Okamoto Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama 1, Higashi-hiroshima 739-8527, Japan. Phone: +81 824 247610. Fax: +81 824 245494Search for more papers by this authorAndrei R. Timerbaev, Andrei R. Timerbaev Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, Kosygin St. 19, 117975 Moscow, Russia Institute of Inorganic Chemistry, Bioinorganic, Environmental and Radiochemistry, Vienna University, Waehringer Str. 42, A-1090, Vienna, AustriaSearch for more papers by this authorTakeshi Hirokawa, Takeshi Hirokawa hiro77@hiroshima-u.ac.jp Search for more papers by this author Hikaru Okamoto, Hikaru Okamoto Department of Applied Chemistry, Graduate School of Engineering, Hiroshima University, 1-4-1 Kagamiyama 1, Higashi-hiroshima 739-8527, Japan. Phone: +81 824 247610. Fax: +81 824 245494Search for more papers by this authorAndrei R. Timerbaev, Andrei R. Timerbaev Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, Kosygin St. 19, 117975 Moscow, Russia Institute of Inorganic Chemistry, Bioinorganic, Environmental and Radiochemistry, Vienna University, Waehringer Str. 42, A-1090, Vienna, AustriaSearch for more papers by this authorTakeshi Hirokawa, Takeshi Hirokawa hiro77@hiroshima-u.ac.jp Search for more papers by this author First published: 04 April 2005 https://doi.org/10.1002/jssc.200400070Citations: 32AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract CE with indirect UV detection was used for the simultaneous determination of lithium, magnesium, calcium, creatinine, carnitine, and a number of amino acids in human serum. The target analytes, positively charged under acidic electrolyte conditions, were separated with positive separation voltage polarity using 10 mM 4-methylbenzylamine, 4.5 mM citric acid, 25% (v/v) methanol at pH 4.05 as background electrolyte providing optimal separation. When analyzing real samples, however, some peaks were broadened due to essentially destacking conditions. In order to maintain the separation efficiency and also enhance the detection sensitivity, transient isotachophoresis (tITP) sample stacking was applied and yielded theoretical plate numbers in the range from 160,000 (arginine) to 350,000 (creatinine). The limit of detection values with tITP preconcentration were 0.11–0.26 mg L–1 for metal cations, 1.0 mg L–1 for creatinine, and 1.3–3.9 mg L–1 for histidine, lysine, arginine, and ornithine. The method precision for peak areas was from 0.4 to 5.0% relative standard deviation using the matrix sodium as internal standard. The accuracy of the developed tITP-CZE system was verified by consistent results for Li+, Mg2+, Ca2+, and creatinine obtained on analyzing two serum certified reference materials. The only sample preparation required was ultrafiltration and acidification (to release protein-bound alkaline earths), and working ranges for individual analytes corresponded well to clinical concentration ranges. Citing Literature Volume28, Issue6April 2005Pages 522-528 RelatedInformation