Candida molischiana β‐Glucosidase Production by Saccharomyces cerevisiae and its Application in Winemaking
2003; Wiley; Volume: 68; Issue: 6 Linguagem: Inglês
10.1111/j.1365-2621.2003.tb07025.x
ISSN1750-3841
AutoresSalvador Genovés, José Vicente Gil, Paloma Manzanares, José Luis Aleixandre, Salvador Vallés,
Tópico(s)Plant Gene Expression Analysis
ResumoABSTRACT: A recombinant Saccharomyces cerevisiae wine yeast strain expressing the Candida molischiana bgln gene encoding a β‐glucosidase (BGLN) has been used to produce this enzyme. Shaking rate, pH, and aeration rate conditions have been optimized to obtain maximum activity to facilitate enzyme purification. The ability of the heterologous enzyme to efficiently release terpenols and alcohols from a Muscat wine glycoside extract and also directly from wine has been demonstrated. Terpenol glycoside content decreased by 50% after 1 mo of wine storage in agreement with results reported for the β‐glucosidase produced by C. molischiana.
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