A silver stain protocol for proteins yielding high resolution and transparent background in sodium dodecyl sulfate‐polyacrylamide gels
1995; Wiley; Volume: 16; Issue: 1 Linguagem: Inglês
10.1002/elps.11501601159
ISSN1522-2683
Autores Tópico(s)Glycosylation and Glycoproteins Research
ResumoA simple, sensitive silver staining method for sodium dodecyl sulfate polyacrylamide gel electrophoresis has been developed which yields high resolution of proteins with a transparent background. The method includes the incorporation of a water wash after a short fixation step in ethanol/ acetic acid and prior to glutaraldehyde cross-linking, which appears to be necessary for the high resolution staining of protein bands and the low background staining.
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