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Noninvasive measurement of midexpiratory flow indicates bronchoconstriction in allergic rats

2002; American Physiological Society; Volume: 93; Issue: 4 Linguagem: Inglês

10.1152/japplphysiol.01121.2001

8750-7587

Thomas Glaab, Heinz G. Hoymann, Jens M. Hohlfeld, Regina Korolewitz, Matthias Hecht, Yves Alarie, Thomas Tschernig, Armin Braun, Norbert Krug, H Fabel,

Allergic Rhinitis and Sensitization

This study was designed to evaluate the value and applicability of tidal breathing pattern analysis to assess bronchoconstriction in conscious rats. Using noninvasive, head-out body plethysmography and the decrease in tidal midexpiratory flow (EF 50 ), we measured airway responsiveness (AR) to inhaled acetylcholine and allergen in conscious Brown-Norway rats, followed by invasive determination of pulmonary conductance (Gl) and EF 50 in anesthetized rats. Dose-response studies to acetylcholine showed that noninvasively recorded EF 50 closely reflected the dose-dependent decreases observed with the invasive monitoring of simultaneously measured Gl and EF 50 . After sensitization and intratracheal boost to ovalbumin or saline, rats were assessed for early and late AR to aerosolized ovalbumin. Ovalbumin aerosol challenge resulted in early and late AR in allergen-sensitized rats, whereas controls were unresponsive. The allergen-specific AR, as measured noninvasively by EF 50 , was similar in degree compared with invasively recorded EF 50 and Gl and was associated with enhanced IgE and airway inflammation. We conclude that EF 50 is a noninvasive and physiologically valid index of bronchoconstriction in a rat model of asthma.