Enumeration of anaerobic and oligotrophic bacteria in subsoils and sediments
1989; Elsevier BV; Volume: 4; Issue: 3 Linguagem: Inglês
10.1016/0169-7722(89)90012-0
ISSN1873-6009
AutoresPatrick van Beelen, Arja K. Fleuren‐Kemilä,
Tópico(s)Methane Hydrates and Related Phenomena
ResumoThe microflora of two sandy subsoils saturated with groundwater, a peat soil, and a river sediment, were enumerated using epifluorescence microscopy and plate counts on rich and diluted media under aerobic and anaerobic conditions. A novel method was used to obtain both aseptic and anaerobic samples. Direct counts revealed high numbers of bacteria per gram dry soil, 2 × 109 and 8 × 107 for the sandy aquifers; 3 × 1010 for the peat soil; and 6 × 1011 for the river sediment. Plate counts on a diluted anaerobic medium showed 2 × 105, 6 × 105, and 6 × 108, respectively, for the latter three soils. The first soil yielded no colonies on various aerobic and anaerobic media and therefore contained less than 104 c.f.u.'s per gram soil. Both the use of aerobic and anaerobic media, or rich and diluted media, gave similar counts. Replica plating of pure strains revealed that the river sediment contained strict anaerobic bacteria whereas the second sandy aquifer contained facultative anaerobes. To the best of our knowledge this is the first paper describing the abundance of bacteria in subsoils and sediments able to grow under oligotrophic and anaerobic conditions.
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