Continuous ethanol fermentation of lactose by a recombinant flocculatingSaccharomyces cerevisiae strain
1999; Wiley; Volume: 64; Issue: 6 Linguagem: Inglês
10.1002/(sici)1097-0290(19990920)64
ISSN1097-0290
AutoresLucı́lia Domingues, Maria M. Dantas, Nelson Lima, J. A. Teixeira,
Tópico(s)Microbial Metabolic Engineering and Bioproduction
ResumoAlcohol fermentation of lactose was investigated using a recombinant flocculating Saccharomyces cerevisiae, expressing the LAC4 (coding for β-galactosidase) and LAC12 (coding for lactose permease) genes of Kluyveromyces marxianus. Data on yeast fermentation and growth on a medium containing lactose as the sole carbon source are presented. In the range of studied lactose concentrations, total lactose consumption was observed with a conversion yield of ethanol close to the expected theoretical value. For the continuously operating bioreactor, an ethanol productivity of 11 g L−1 h−1 (corresponding to a feed lactose concentration of 50 g L−1 and a dilution rate of 0.55 h−1) was obtained, which is 7 times larger than the continuous conventional systems. The system stability was confirmed by keeping it in operation for 6 months. © 1999 John Wiley & Sons, Inc. Biotechnol Bioeng 64: 692–697, 1999.
Referência(s)