Noggin overexpression inhibits eyelid opening by altering epidermal apoptosis and differentiation
2003; Springer Nature; Volume: 22; Issue: 12 Linguagem: Inglês
10.1093/emboj/cdg291
ISSN1460-2075
AutoresAndrey A. Sharov, Lorin Weiner, Tatyana Sharova, Frank Siebenhaar, Ruzanna Atoyan, Anthony M. Reginato, Coleen A. McNamara, Keiko Funa, Barbara A. Gilchrest, Janice L. Brissette, Vladimir A. Botchkarev,
Tópico(s)melanin and skin pigmentation
ResumoArticle16 June 2003free access Noggin overexpression inhibits eyelid opening by altering epidermal apoptosis and differentiation Andrei A. Sharov Andrei A. Sharov Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Lorin Weiner Lorin Weiner Cutaneous Biology Research Center, Harvard Medical School, Charlestown/Boston, MA, 02115 USA Search for more papers by this author Tatyana Y. Sharova Tatyana Y. Sharova Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Frank Siebenhaar Frank Siebenhaar Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Ruzanna Atoyan Ruzanna Atoyan Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Anthony M. Reginato Anthony M. Reginato Department of Cell Biology, Harvard Medical School, Charlestown/Boston, MA, 02115 USA Search for more papers by this author Coleen A. McNamara Coleen A. McNamara Cardiovascular Division, University of Virginia Health Care Center, Charlottesville, VA, 22908 USA Search for more papers by this author Keiko Funa Keiko Funa Department of Cell Biology, Goteborg University, Goteborg, Sweden Search for more papers by this author Barbara A. Gilchrest Barbara A. Gilchrest Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Janice L. Brissette Janice L. Brissette Cutaneous Biology Research Center, Harvard Medical School, Charlestown/Boston, MA, 02115 USA Search for more papers by this author Vladimir A. Botchkarev Corresponding Author Vladimir A. Botchkarev Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Andrei A. Sharov Andrei A. Sharov Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Lorin Weiner Lorin Weiner Cutaneous Biology Research Center, Harvard Medical School, Charlestown/Boston, MA, 02115 USA Search for more papers by this author Tatyana Y. Sharova Tatyana Y. Sharova Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Frank Siebenhaar Frank Siebenhaar Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Ruzanna Atoyan Ruzanna Atoyan Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Anthony M. Reginato Anthony M. Reginato Department of Cell Biology, Harvard Medical School, Charlestown/Boston, MA, 02115 USA Search for more papers by this author Coleen A. McNamara Coleen A. McNamara Cardiovascular Division, University of Virginia Health Care Center, Charlottesville, VA, 22908 USA Search for more papers by this author Keiko Funa Keiko Funa Department of Cell Biology, Goteborg University, Goteborg, Sweden Search for more papers by this author Barbara A. Gilchrest Barbara A. Gilchrest Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Janice L. Brissette Janice L. Brissette Cutaneous Biology Research Center, Harvard Medical School, Charlestown/Boston, MA, 02115 USA Search for more papers by this author Vladimir A. Botchkarev Corresponding Author Vladimir A. Botchkarev Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA Search for more papers by this author Author Information Andrei A. Sharov1, Lorin Weiner2, Tatyana Y. Sharova1, Frank Siebenhaar1, Ruzanna Atoyan1, Anthony M. Reginato3, Coleen A. McNamara4, Keiko Funa5, Barbara A. Gilchrest1, Janice L. Brissette2 and Vladimir A. Botchkarev 1 1Department of Dermatology, Boston University School of Medicine, 609 Albany Street, Boston, MA, 02118 USA 2Cutaneous Biology Research Center, Harvard Medical School, Charlestown/Boston, MA, 02115 USA 3Department of Cell Biology, Harvard Medical School, Charlestown/Boston, MA, 02115 USA 4Cardiovascular Division, University of Virginia Health Care Center, Charlottesville, VA, 22908 USA 5Department of Cell Biology, Goteborg University, Goteborg, Sweden *Corresponding author. E-mail: [email protected] The EMBO Journal (2003)22:2992-3003https://doi.org/10.1093/emboj/cdg291 PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Contact of developing sensory organs with the external environment is established via the formation of openings in the skin. During eye development, eyelids first grow, fuse and finally reopen, thus providing access for visual information to the retina. Here, we show that eyelid opening is strongly inhibited in transgenic mice overexpressing the bone morphogenetic protein (BMP) antagonist noggin from the keratin 5 (K5) promoter in the epidermis. In wild-type mice, enhanced expression of the kinase-inactive form of BMPR-IB mediated by an adenovirus vector also inhibits eyelid opening. Noggin overexpression leads to reduction of apoptosis and retardation of cell differentiation in the eyelid epithelium, which is associated with downregulation of expression of the apoptotic receptors (Fas, p55 kDa TNFR), Id3 protein and keratinocyte differentiation markers (loricrin, involucrin). BMP-4, but not EGF or TGF-α, accelerates opening of the eyelid explants isolated from K5-Noggin transgenic mice when cultured ex vivo. These data suggest that the BMP signaling pathway plays an important role in regulation of genetic programs of eyelid opening and skin remodeling during the final steps of eye morphogenesis. Introduction Skin morphogenesis is a complex process resulting not only in an organ that covers and protects the body (Fuchs and Raghavan, 2002), but also in the formation of openings overlying sensory organs, such as the eye or ear, to provide access for environmental signals to sensory epithelia. During eye development, the skin forms eyelids, which first grow and fuse, thus covering corneal epithelium, and then reopen at a certain developmental stage when the eye becomes morphologically and functionally mature to accept visual signals (Findlater et al., 1993). The process of eyelid opening requires a high degree of coordination between cell proliferation, apoptosis and differentiation (Teraishi and Yoshioka, 2001). While signaling through epidermal growth factor receptors (EGFRs) accelerates eyelid opening (Cohen, 1962; Smith et al., 1985; Tam, 1985), other signaling pathways that control this process remain poorly understood. Bone morphogenetic proteins (BMPs) play pivotal roles in the control of morphogenesis of the skin, as well as in eye development: BMP-4 induces epidermal cell fate in the early embryo, and later together with BMP-7 regulates lens formation and ciliary body development (Hogan, 1996, 1999; Zhao et al., 2002). BMPs also regulate morphogenesis of skin appendages (Blessing et al., 1993; Noramly and Morgan, 1998; Botchkarev et al., 1999; Kulessa et al., 2000; Yu et al., 2002). Noggin is a secreted protein that modulates the activity of BMP-2/4/7 and growth differentiation factor-5 (GDF-5) in vivo by preventing their interaction with BMP receptors (Zimmerman et al., 1996; Miyazono et al., 2001; Groppe et al., 2002). This modulation is critically important for proper orchestration of a large variety of developmental events (Hogan, 1999; Massagué and Chen, 2000). Indeed, noggin knockout mice show multiple defects in skeletal and nervous system development, leading to their lethality shortly prior to birth (Brunet et al., 1998; McMahon et al., 1998). BMPs exert their biological effects by binding to specific BMP receptor complexes, which transduce the signal to the nucleus via the BMP-Smad and BMP-MAP kinase pathways (von Bubnoff and Cho, 2001). Both pathways are implicated in the regulation of cell proliferation, differentiation and apoptosis during embryonic development (reviewed in Hogan, 1996, 1999). Using a transgenic mouse approach, it was shown that BMP-4 and noggin also play important roles in the regulation of cell differentiation and apoptosis during postnatal development (Blessing et al., 1993; Kulessa et al., 2000; Guha et al., 2002). However, the in vivo functions of noggin in the regulation of eyelid opening remain unclear. Here, we show that transgenic (TG) mice overexpressing BMP-antagonist noggin under the control of the keratin 5 (K5) promoter display severe inhibition of eyelid opening accompanied by alterations of both apoptosis and cell differentiation in the eyelid epithelium. We also show that in wild-type (WT) mice, enhanced expression of a kinase-inactive form of BMPR-IB mediated by an adenovirus vector inhibits eyelid opening. Furthermore, we demonstrate that the inhibition of eyelid opening seen in K5-Noggin mice is associated with downregulation of the apoptotic receptors (Fas, p55 kD TNFR), Id3 protein and keratinocyte (KC) differentiation markers (loricrin, involucrin) in the eyelid epithelium. Finally, we show in ex vivo experiments that BMP-Smad and EGFR signaling operate as two parallel pathways in regulating eyelid opening, suggesting important roles for BMPs and noggin in controlling skin remodeling during the final steps of eye morphogenesis. Results Noggin-overexpressing mice show severe retardation of eyelid opening To explore the roles of BMP-2/4/7 and GDF-5 and their antagonist noggin in the regulation of skin remodeling during eyelid opening, TG mice expressing noggin in the basal epidermal KCs under the control of the K5 promoter were generated (Figure 1). The noggin cDNA containing a 5′ flag epitope was inserted into a targeting vector containing the human K5 promoter (Figure 1A). Two TG founder lines were generated using C3H/HeJ mice as a background strain. Both lines of TG mice were viable, fertile and showed a weight similar to WT mice at postnatal day 0.5 (P0.5), while showing significantly (P < 0.05) retarded weight gain thereafter (15.3 ± 1.7 g versus 19.7 ± 2.1 g seen in WT mice at P30.5). Figure 1.Creation of transgenic mice overexpressing noggin in the epidermis. (A) Scheme of the transgene construct. (B and D) Inhibition of eyelid opening observed in transgenic (TG) mice at P13.5 and P34.5. (C and E) Microscopy of eyelids in WT and TG mice. (F and G) Expression of Flag in the EJE of TG mice (G, arrows) using antiserum against Flag protein. Lack of expression in WT mice (F). (H) Alkaline phosphatase activity in murine osteoblasts induced by BMP-4 after incubation with media isolated from cultured TG or WT keratinocytes (KCs), or with purified noggin protein. (I) Western blot analysis of 64 kDa noggin protein detected by antisera to full-length mouse noggin or to the Flag protein tagged to the uncleaved noggin in lysates of WT and TG eyelid skin at P0.5. (J and K) Immunofluorescence with antiserum against noggin shows expression in the EJE, the dermis and the corneal epithelium of TG mice (K, arrows, small and large arrowheads, respectively), and in WT mice (J) at P10.5. (L and M) Histological images of dorsal skin of newborn WT and TG mice. Lack of granular layer in the TG epidermis (M, arrows), compared with the WT epidermis (L, arrows). (N and O) Immuno-visualization of Ki67 (red fluorescence) and TUNEL (green fluorescence) in dorsal skin of WT and TG at P7.5. Proliferating cells in the epidermis and dermis are indicated by arrows and arrowheads, respectively. Epidermal/dermal and dermal/subcutaneous borders are indicated by dotted lines. C, cornea; LE and UE, lower and upper eyelids, respectively. Scale bars: 50 μm. Download figure Download PowerPoint Both lines of TG mice had markedly delayed eyelid opening. While in WT mice, eyelids were open by P13.5, K5-Noggin mice showed a delay in eyelid opening of 17–24 days (average 20.5 days; Figure 1B–E). The process of eyelid opening was equally retarded in both male and female TG mice. No visible abnormalities in corneal development were seen in K5-Noggin mice (Figure 1C and E). In contrast to eyelid development, K5-Noggin mice showed no macroscopic abnormalities in external auditory canal formation or limb development compared with WT mice. Abnormalities in hair follicle development and cycling observed in TG mice will be described elsewhere. Transgene expression was monitored by immunofluorescent Flag visualization. K5-Noggin mice showed strong transgene expression in the epidermal basal layer of the eyelid junction, while no specific signal was seen in WT skin (Figure 1F and G). To identify whether transgene-derived noggin protein is able to neutralize BMP activity, we tested the capacity of the medium derived from cultured transgenic KCs (or from WT KCs as a control) to neutralize BMP-4-induced osteoblast differentiation. Osteoblast differentiation was monitored by analyzing alkaline phosphatase activity (Aoki et al., 2001). Our data suggest that the medium derived from TG KCs significantly (P < 0.05) reduced alkaline phosphatase activity in osteoblasts induced by BMP-4, similar to that seen after the addition of purified noggin protein to osteoblasts incubated with BMP-4 (Figure 1H). In contrast, the medium derived from WT KCs failed to significantly reduce alkaline phosphatase activity induced by BMP-4 in these cells. These data suggest that the transgene-derived noggin retains BMP-inhibitory activity similar to WT noggin protein. To determine whether TG mice show an increase of noggin protein in skin, western blot analysis and immunofluorescent stainings were preformed. By western blotting, high levels of 64 kDa noggin protein, determined using anti-noggin or anti-flag antisera, were detected in the eyelid skin of TG mice (Figure 1I). In contrast to TG skin, the lower levels of noggin expression or lack of any signal were detected in WT skin lysates after using the anti-noggin and anti-flag antisera, respectively (Figure 1I). Compared with WT mice (Figure 1J), noggin expression was increased in the eyelid junction epithelium (EJE) and in the dermis of TG mice (Figure 1K). Newborn TG mice showed a lack of the granular layer in the epidermis (Figure 1L and M). However, a clear granular layer was visible in TG mice by 7–8 days. At P7.5, when the major morphogenetic steps in skin development are already complete, TG mice showed a significantly (P < 0.01) increased thickness of the epidermis (42.4 ± 5.6 μm) and dermis (171.8 ± 19.5 μm) compared with the corresponding parameters of WT mice (24.9 ± 6.5 and 103.8 ± 17.3 μm, respectively). These changes, however, were not accompanied by significant alterations in epidermal proliferation or apoptosis compared with WT skin (Figure 1N and O). All key components of the BMP signaling pathway (noggin, BMP-2/4/7, GDF-5, BMP receptors, Smad1/5 proteins) are expressed in developing eyelid epithelium To investigate the role of BMP signaling in skin remodeling during eyelid opening, we examined the expression patterns of noggin, BMP-2/4/7, GDF-5, BMP receptors and Smad1/5 in murine eyelid skin between embryonic day 18.5 (E18.5) and P13.5; times when eyelids are fully closed and open, respectively (Findlater et al., 1993). At E18.5, noggin knockout (+/) mice with a lacZ gene targeted to the noggin locus (McMahon et al., 1998) showed strong expression of noggin in both the epithelium and mesenchyme of the lower eyelid, while relatively weak expression was observed in the mesenchyme of the upper eyelid (Figure 2A). In WT mice, BMP-2 and BMP-4 mRNAs were detected in single cells of the EJE, cornea and dermis at P0.5 (Figure 2B and C), and later at P7.5–P10.5 (data not shown). Figure 2.Expression patterns for noggin, BMP-2 and BMP-4 mRNAs, BMP-7, GDF-5, BMP receptors and Smad1/5 proteins during eyelid opening. Cryo-sections of E18.5 murine embryos and P0.5–P10.5 postnatal mice were processed for microscopic visualization of noggin, BMP-2/4 mRNAs, BMP-7, GDF-5, BMPR-IA/IB and Smad1/5. (A) Noggin. LacZ activity in the epithelium of the lower eyelid (arrow) and in the mesenchyme of the upper and lower eyelids (arrowheads) of noggin knockout (+/−) mice. (B–D) BMP-2/4 mRNAs. In situ hybridization signals in basal and suprabasal cells of the eyelid junction (arrows and large arrowheads, respectively), and in corneocytes (small arrowheads) at P0.5. Sense control for BMP-4 mRNA is shown in (D). Melanin granules in (C) and (D) are shown by arrows. (E and G) BMP-7. Expression in basal (E, arrows) and differentiating cells (G, arrows) of the EJE at P0.5 (E) and P10.5 (G). (F and H) GDF-5. Expression in the eyelid mesenchyme (arrowheads) and in basal cells of the EJE (arrows) at P0.5 (F) and P10.5 (H). (I and M) BMPR-IA. Expression in basal and differentiating cells of the EJE, and in corneocytes (arrows, large and small arrowheads, respectively) at P0.5 (I) and P10.5 (M). (J and N) BMPR-IB. Lack of expression in the eyelids at P0.5 (J, arrows). Expression in basal and suprabasal cells of the EJE at P10.5 (N, large and small arrows, respectively). (K and O) Smad1. Nuclear expression in basal and suprabasal cells of the EJE and in corneocytes (large and small arrows and arrowheads, respectively) at P0.5 (K) and P10.5 (O). (L and P) Smad5. Lack of nuclear expression in the eyelids at P0.5 (L). Expression in the basal and suprabasal cells of the EJE and in corneocytes at P10.5 (P, large and small arrows and arrowheads, respectively). C, cornea; LE and UE, lower and upper eyelids, respectively; Mel, melanin. In (B–D), a border between the EJE and the dermis is indicated by dotted line. Scale bars: 50 μm. Download figure Download PowerPoint Only faint expression of BMP-7 and GDF-5 proteins was observed in eyelids at P0.5 (Figure 2E and F), while at P10.5 an increase of BMP-7 and GDF-5 immunoreactivity was found in the differentiating and basal cells of the EJE, respectively (Figure 2G and H). Both BMPR-IA and Smad1 proteins were expressed in the EJE and cornea of newborn mice (Figure 2I and K), while no BMPR-IB expression was observed at this time (Figure 2J). At P0.5, strong cytoplasmic and nuclear expression of Smad1 was found in basal and suprabasal cells of the EJE (Figure 2K), while only weak cytoplasmic Smad5 expression was seen in the EJE (Figure 2L). At P10.5, BMPR-IA and Smad1 proteins were expressed in differentiating cells of the EJE (Figure 2M and O), while BMPR-IB and Smad-5 were seen in both basal and suprabasal KCs (Figure 2N and P). Most importantly, both Smad-1 and Smad-5 showed nuclear patterns of expression in suprabasal cells of the EJE at P10.5 (Figure 2O and P), suggesting their involvement in the regulation of cell differentiation. Noggin transgenic mice show reduced apoptosis in developing eyelid epithelium To analyze the mechanisms that are involved in the control of eyelid opening by BMPs and noggin, we first compared the dynamics of cell proliferation and apoptosis in the eyelid epithelium between WT and TG mice. No significant difference (P > 0.05) between the number of Ki-67+ cells in the EJE was found between WT and TG mice at P0.5 (Figure 3A and B), or at later times (P7.5–P13.5; data not shown). In WT mice, maximal apoptosis detected by TUNEL was observed in the triangle between the inner epithelial portions of the upper and lower eyelids and cornea at P0.5 (Figure 3A). However, newborn TG mice showed a significant reduction in the number of TUNEL+ cells in this area of the eyelid epithelium, compared with WT mice (4.7 ± 0.6 per microscope field versus 13.5 ± 2.4 per microscope field in WT mice, P < 0.01; Figure 3B). In WT mice, TUNEL+ cells became visible in the EJE at P1.5 and quickly disappeared from this area by P2.5, while TUNEL+ cells were still visible in the EJE of TG mice up to P7.5 (data not shown). Figure 3.Alterations in expression of the apoptotic markers in K5-Noggin mice. Eyelid skin was isolated from newborn WT or transgenic (TG) mice and processed for immunofluorescence (A–F, I–T), in situ hybridization (G, H) or semi-quantitative RT–PCR (U) protocols for analyses of the expression of apoptotic markers. (A and B) TUNEL (green) plus Ki67 (red). Marked decrease of TUNEL+ cells in TG mice (B, labeled area) compared with WT mice (A, labeled area). (C and D) TAK1. Lack of expression in the EJE of both WT and TG mice. (E and F) p21. Strong expression in basal and suprabasal eyelid keratinocytes (KCs) (large and small arrows, respectively). (G and H) Msx-2. Lack of in situ hybridization signal in the EJE and weak expression in the corneal epithelium (arrowheads). The border between eyelid epithelium and mesenchyme is indicated by the dotted lines. (I and J) pSmad1/5. Decrease of nuclear pSmad1/5 expression in the EJE (J, labeled area) and mesenchyme (J, arrowheads) of TG mice compared with the same structures in WT mice (I). (K and L) Id3. Decrease of expression in the EJE of TG mice (L, arrows) compared with WT mice (K). (M and N) p75NTR. Expression in mesenchyme around developing hair follicles (arrows). (O and P) Edar. Expression in the epithelium of developing hair follicles (arrows). (Q and T) Fas and p55TNFR. Marked decrease of expression in the eyelids of TG mice (R, T, arrows), compared with WT mice (Q, S, arrows). (U) Semi-quantitative RT–PCR. Eyelid skin dissected from WT and K5-Noggin TG mice was processed for semi- quantitative RT–PCR of β-actin, p21, Id1, Id2, Id3, p75NTR, Fas and p55TNFR. Representative gels from one of three experiments each are shown. C, cornea; LE and UE, lower and upper eyelids, respectively. Scale bars: (A–D) and (I–T), 50 μm; (E–F), 25 μm. Download figure Download PowerPoint Next, the expression patterns for markers implicated in BMP-mediated apoptosis (XIAP, TAK1 kinase, Msx-2 transcription factor and cyclin-dependent kinase inhibitor p21; von Bubnoff and Cho, 2001) were compared between eyelids of WT and TG mice. However, no expression of TAK1 kinase (Figure 3C and D) and XIAP (data not shown) was detected in the EJE of either WT or TG mice at P0.5–P10.5. The p21 protein showed similar expression patterns in nuclei of differentiating cells of WT and TG mice (Figure 3E and F). No differences in the expression levels of p21 transcripts were found between WT and TG skin at P0.5 or P10.5 (Figure 3U). No expression of Msx-2 mRNA was seen in the EJE of WT and TG mice at P0.5 (Figure 3G and H) or P7.5 (data not shown). These data suggest that XIAP, TAK1, p21 and Msx-2 most likely do not contribute to the alterations of apoptosis observed in the eyelid epithelium of TG mice. Inhibition of apoptosis in K5-Noggin mice is associated with downregulation of pSmad1/5, Id3 protein and apoptotic receptors (Fas, p55TNFR) in the eyelid epithelium The BMP-Smad pathway has also been implicated in apoptosis regulation by controlling the expression of BMP target genes (von Bubnoff and Cho, 2001). In newborn WT mice, strong nuclear expression of pSmad1/5 was found in cells located in the triangle between the inner epithelial portions of the upper and lower eyelids and cornea (Figure 3I), i.e. in the area where maximal apoptosis was seen (Figure 3A). However, in newborn TG mice, only weak cytoplasmic pSmad1/5 expression was detected in cells of this area and in other cells of the EJE (Figure 3J). This suggests that Smad1/5 phosphorylation and nuclear translocation are both decreased in TG mice, which implicates Smad involvement in mediating BMP-induced apoptosis during eyelid opening. Id proteins (inhibitors of DNA binding/differentiation) and the p75 kD neurotrophin receptor (p75NTR) are important targets for BMP regulation and are also capable of inducing apoptosis (Botchkarev et al., 1999; Yokota, 2001). To examine the possible involvement of Id proteins and ‘death-domain’ receptors in BMP-mediated apoptosis during eyelid opening, the expression of Id1/2/3, p75NTR, p55 kD TNF receptor (p55TNFR), Fas and the ectodermal dysplasia receptor (Edar) in the eyelid epithelium was compared between WT and TG mice at P0.5–P10.5. Among different members of the Id family, only the Id3 protein and its transcripts were expressed in the developing eyelid epithelium of newborn WT mice (Figure 3K and U). Only weak expression of Id1/2 transcripts was seen in both WT and TG eyelid skin at P0.5 (Figure 3U), while no Id1/Id2 protein expression was evident by immunofluorescence (data not shown). In contrast to WT mice, Id3 protein and mRNA expression were markedly decreased in eyelids of TG mice at P0.5–P10.5 (Figure 3L and U). p75NTR and Edar expression was not significantly different between WT and TG mice (Figure 3M–P), but the expression of the Fas and p55TNFR proteins and their mRNAs was strongly reduced in the eyelids of newborn TG mice compared with WT mice (Figure 3Q–T and U). In contrast to WT mice, the onset of expression of both Fas and p55TNFR proteins in the EJE of TG mice was delayed by 7–9 days, and at P10.5 lower levels of both Fas and p55TNFR transcripts were present in the eyelids of TG mice, compared with WT animals (Figure 3U). These data suggest that BMPs may promote apoptosis in the developing eyelid epithelium by regulating the expression of Id3, Fas and p55TNFR. K5-Noggin mice show alterations in keratinocyte differentiation during eyelid epithelium development To examine whether the inhibition of eyelid opening seen in TG mice may also be due to the alterations in cell differentiation, the dynamics of epidermal keratins and KC differentiation markers in eyelids were compared between WT mice and TG mice at P0.5–P10.5. Abundant amounts of K5 were present in basal epidermal KCs of back skin and eyelid skin in both WT and TG mice (Figure 4A, B, Q and R). However, the level of K14 was significantly decreased in the eyelids of both WT and TG mice compared with back skin (Figure 4C, D, S and T). Instead, K15 was present in basal and suprabasal KCs of eyelid skin in WT and TG mice, while it was lacking in the epidermis of back skin (Figure 4E, F, U and V). This result was confirmed by western blot analysis, which showed a marked decrease of K14 protein and an increase of K15 in the lysates of eyelid skin compared with back skin (Figure 4W). Figure 4.Differences in the expression of keratinocyte differentiation markers in eyelid epithelium between WT and K5-Noggin mice. Eyelid and back skin was isolated from WT or TG mice at P7.5–P10.5 and processed for analyses of the expression of keratinocyte (KC) markers, BMP-7, GDF-5 and pSmad1/5 using immunofluorescence (A–V), western blot analysis (W) or semi-quantitative RT–PCR (X). (A and B) and (Q and R): keratin 5. Expression in basal KCs (arrows) of the EJE (A and B) and in back skin (Q and R). (C and D) and (S and T): keratin 14. Decrease of expression (arrows) in the EJE (C and D) compared with back skin (S and T). (E and F) and (U and V): keratin 15. Marked increase of expression (arrows) in the EJE (E and F) compared with back skin (U and V). (G–J) Keratin 10 (G and H) and filaggrin (I and J). Expression in suprabasal KCs of the EJE (arrows). (K–N) Involucrin (K and L) and loricrin (M and N). Marked decrease of expression in the EJE of TG mice compared with WT mice (arrows). (O and P) pSmad1/5. Decrease of pSmad1/5 expression in the basal and suprabasal KCs and in eyelid mesenchyme (P, large arrows, small arrows and arrowheads, respectively) of TG mice compared with the same structures of WT mice (O). Scale bars for (A–L) are 50 μm. (W) Western blot analysis of the K5 (60 kDa), K14 (55 kDa) and K15 (49 kDa) proteins in lysates of the WT eyelid and back skin at P10.5. (X) Semi-quantitative RT–PCR. Eyelid skin dissected from WT and TG mice at P0.5 and P10.5 was processed for semi-quantitative RT–PCR with primers specific for β-actin, filaggrin, involucrin, loricirn, BMP-7 and GDF-5. Representative gels from one of three experiments each are shown. Download figure Download PowerPoint In eyelid skin, no differences in K10 and filaggrin protein levels were evident between WT and TG mice at P10.5 (Figure 4G–J and X), when all differentiation markers examined showed maximal expression. However, the amounts of involucrin and loricrin proteins and their corresponding transcripts were strongly reduced in eyelids of TG mice at P10.5 compared with WT mice (Figure 4K–N and X). No differences in the transcript levels of BMP-7 and GDF-5, which may regulate keratinocyte differentiation in the EJE, were found between WT and TG eyelids at P10.5 (Figure 4X). In contrast to WT mice, a marked decrease of pSmad1/5 expression was evident in the EJE of TG mice at P7.5 (Figure 4O and P). Thus, these data suggest that the reduced amount of K14 is partially replaced and compensated for by K15 in the developing eyelids of both WT and TG mice. Reduced expression of involucrin and loricrin in TG eyelids suggests that they may be targets for BMP-Smad regulation during eyelid development. Enhanced expression of a kinase-inactive form of BMPR-IB mediated by an adenovirus vector inhibits eyelid opening and keratinocyte differentiation in WT mice BMP-induced cell differentiation is mediated by both BMPR-IA and BMPR-IB (reviewed in von Bubnoff and Cho, 2001; Botchkarev, 2003). To determine which BMP receptor subtypes are involved in the control of cell differentiation in developing eyelid
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