Artigo Revisado por pares

Characterization of Dental Epithelial Progenitor Cells Derived from Cervical-loop Epithelium in a Rat Lower Incisor

2004; SAGE Publishing; Volume: 83; Issue: 2 Linguagem: Inglês

10.1177/154405910408300209

ISSN

1544-0591

Autores

Shinji Kawano, M. Saito, Keisuke Handa, Takahiko Morotomi, Takashi Toyono, Yuji Seta, N. Nakamura, Takashi Uchida, Kuniaki Toyoshima, Masanobu Ohishi, Hidemitsu Harada,

Tópico(s)

Periodontal Regeneration and Treatments

Resumo

Dental epithelial progenitor cells differentiate into various cell types during development of tooth germs. To study this mechanism, we produced immortalized dental epithelial progenitor cells derived from the cervical-loop epithelium of a rat lower incisor. The expression patterns of cytokeratin 14, nerve growth factor receptor p75, amelogenin, Notch2, and alkaline phosphatase were examined by immnohistochemistry in both lower and higher cell densities. The patterns of each were compared in the dental epithelium of rat lower incisors. The results demonstrated that these cells could produce ameloblast lineage cells, stratum intermedium cells, stellate reticulum, and outer enamel epithelium. Furthermore, fibroblast growth factor 10 stimulated proliferation of dental progenitor cells and subsequently increased the number of cells expressing alkaline phosphatase. These results suggest that fibroblast growth factor 10 plays a role in coupling mitogenesis of the cervical-loop cells and the production of stratum intermedium cells in rat incisors.

Referência(s)